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C. L. Walden, W. Shen, J. G. Sivak, B. J. McConkey, T. D. Singer; Proteomic Studies of Myopia Induced With Minus Lenses in Tilapia. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1744.
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To expand the usefulness of Tilapia (Oreochromis niloticus) as a model organism to investigate refractive development. Here we use a novel proteomics screening protocol to identify potentially important protein biomolecules associated with myopia induced with minus lenses.
-12D lenses were directly sutured over one eye for one-day, four-days, ten-days and ten-day plus two day recovery time intervals to mimic early to late refractive error development as well as the recovery from the myopic state. 2-D Differential in Gel Electrophoresis (DIGE) was used in conjunction with Mass Spectrometry (MS) to identify proteins that were differentially expressed in the retina/choroid complex.
Treated fish eyes showed significant amounts of induced myopia. Two- dimensional Differential in Gel Electrophoresis (2D DIGE) was performed to isolate proteins that were differentially expressed in myopic retina and choroid tissues versus untreated tissues. A total of 38 isolated protein spots had statistically significant differential expression over the course of the experiment. Eight of the differentially expressed proteins were identified by mass spectrometry.
The use of a proteomic screening protocol has identified potential myopia related targets and provided insight into the molecular mechanisms of myopia. It was found that creatine kinase, glyceraldehyde-3-phosphate dehydrogenase and lactate dehydrogenase b showed promise as metabolic biomolecules involved in the process of myopic development. Further characterization is currently in progress.
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