May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Proteomic Studies of Myopia Induced With Minus Lenses in Tilapia
Author Affiliations & Notes
  • C. L. Walden
    University of Waterloo, Waterloo, Ontario, Canada
    School of Optometry,
  • W. Shen
    University of Waterloo, Waterloo, Ontario, Canada
    School of Optometry,
  • J. G. Sivak
    University of Waterloo, Waterloo, Ontario, Canada
    School of Optometry,
    Department of Biology,
  • B. J. McConkey
    University of Waterloo, Waterloo, Ontario, Canada
    Department of Biology,
  • T. D. Singer
    University of Waterloo, Waterloo, Ontario, Canada
    School of Optometry,
    Department of Biology,
  • Footnotes
    Commercial Relationships  C.L. Walden, None; W. Shen, None; J.G. Sivak, None; B.J. McConkey, None; T.D. Singer, None.
  • Footnotes
    Support  CNIB E.A. Baker to TDS, Natural Sciences and Engineering Research Council of Canada to CLW, JGS, JBM and TDS
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 1744. doi:https://doi.org/
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      C. L. Walden, W. Shen, J. G. Sivak, B. J. McConkey, T. D. Singer; Proteomic Studies of Myopia Induced With Minus Lenses in Tilapia. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1744. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To expand the usefulness of Tilapia (Oreochromis niloticus) as a model organism to investigate refractive development. Here we use a novel proteomics screening protocol to identify potentially important protein biomolecules associated with myopia induced with minus lenses.

Methods: : -12D lenses were directly sutured over one eye for one-day, four-days, ten-days and ten-day plus two day recovery time intervals to mimic early to late refractive error development as well as the recovery from the myopic state. 2-D Differential in Gel Electrophoresis (DIGE) was used in conjunction with Mass Spectrometry (MS) to identify proteins that were differentially expressed in the retina/choroid complex.

Results: : Treated fish eyes showed significant amounts of induced myopia. Two- dimensional Differential in Gel Electrophoresis (2D DIGE) was performed to isolate proteins that were differentially expressed in myopic retina and choroid tissues versus untreated tissues. A total of 38 isolated protein spots had statistically significant differential expression over the course of the experiment. Eight of the differentially expressed proteins were identified by mass spectrometry.

Conclusions: : The use of a proteomic screening protocol has identified potential myopia related targets and provided insight into the molecular mechanisms of myopia. It was found that creatine kinase, glyceraldehyde-3-phosphate dehydrogenase and lactate dehydrogenase b showed promise as metabolic biomolecules involved in the process of myopic development. Further characterization is currently in progress.

Keywords: myopia • proteomics • retina 
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