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K. C. Leonard, C. M. L. Hutnik, T. A. Newson, D. W. Holdsworth, D. O'Carroll, J. Barfett, K. A. Hill; Modelling Drug and Fluid Transfer Through the Porcine and Lapine Eye. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1819.
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Topical administration is the least invasive and preferred method of delivery for most ocular medications. Thus, predicting the delivery of drugs from the cornea to all major ocular structures, especially the retina, is of great interest. The goal of the current project is to model the transport of a drug from the cornea or anterior chamber to various therapeutic targets within the eye utilizing MicroCT. A computational fluid dynamics (CFD) model of flow patterns within the eye will then be created, which can be used to predict delivery of drugs to various sites in the eye.
Twelve cadaveric porcine and four lapine eyes were injected with Iohexol, a low-osmolar non-ionic iodinated contrast agent, into the anterior chamber or vitreous. Varying initial volumes (0.01, 0.02 and 0.03 ml) and concentrations (12.5, 25 and 50%) of Iohexol were injected into the different eyes in order to identify optimal injection parameters. A conebeam micro-CT scanner was used to acquire 3D CT image data. CT images were acquired at 12, 22, 32, 52 and 72 min. CT data were analyzed with dedicated 3D visualization and analysis software which allowed the specimens to be viewed in multi-planar reformatted views, showing cross-sections through transverse, sagittal, and coronal planes.
Optimal injection parameters for tracking the movement of CT contrast were identified for the anterior chamber and vitreous. For injections administered into the vitreous a volume of 0.03 ml of the 50% Iohexol dilution (150 mg/ml iodine content) was determined to be optimal. A volume of 0.01 ml of the 50% dilution was optimal for anterior chamber injections. The capability of generating 3D figures outlining the precise movement of contrast from the injection location has also been established.
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