May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
In vivo Retinal Histology of Shields Gray Crescents by Spectral Domain Optical Coherence Tomography (SD-OCT)
Author Affiliations & Notes
  • R. S. Rajpara
    Bascom Palmer Eye Insitute, U. of Miami Miller School of Medicine, Miami, Florida
  • K. Whipple
    Bascom Palmer Eye Insitute, U. of Miami Miller School of Medicine, Miami, Florida
  • A. G. Smith
    Bascom Palmer Eye Insitute, U. of Miami Miller School of Medicine, Miami, Florida
  • G. Gregori
    Bascom Palmer Eye Insitute, U. of Miami Miller School of Medicine, Miami, Florida
  • R. W. Knighton
    Bascom Palmer Eye Insitute, U. of Miami Miller School of Medicine, Miami, Florida
  • R. K. Lee
    Bascom Palmer Eye Insitute, U. of Miami Miller School of Medicine, Miami, Florida
  • D. R. Anderson
    Bascom Palmer Eye Insitute, U. of Miami Miller School of Medicine, Miami, Florida
  • Footnotes
    Commercial Relationships  R.S. Rajpara, None; K. Whipple, None; A.G. Smith, None; G. Gregori, Carl Zeiss Meditec, Inc., F; University of Miami, P; R.W. Knighton, Carl Zeiss Meditec, Inc., F; University of Miami, P; R.K. Lee, None; D.R. Anderson, None.
  • Footnotes
    Support  NIH K08 EY016775 (RKL), American Health Assistance Foundation (RKL) and Research to Prevent Blindness and NIH Core grant P30-EY014801 to the U. of Miami and in part by a grand from Carl Zeiss Meditec.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 1866. doi:
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      R. S. Rajpara, K. Whipple, A. G. Smith, G. Gregori, R. W. Knighton, R. K. Lee, D. R. Anderson; In vivo Retinal Histology of Shields Gray Crescents by Spectral Domain Optical Coherence Tomography (SD-OCT). Invest. Ophthalmol. Vis. Sci. 2008;49(13):1866.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Shields characterized temporal optic nerve head (ONH) "gray crescents" (AJO 89:238 (1980), a dark region that makes it difficult to judge ONH cupping. Subsequently, it became necessary to clarify that this structure was different from a different type of dark tissue at the curved disc boundary, the former designated type A and the latter, type B. The histological characteristics of these are here characterized from images obtained by spectral domain ocular coherence tomography (SD-OCT).

Methods: : Digital color photographs were taken with a Nidek simultaneous stereo camera and images recorded by SD-OCT (prototype of Cirrus HD-OCT, Carl Zeiss Meditec, Inc.). The en face SD-OCT images were registered with the ONH photographs. Cross-sectional B-scan images across the areas of gray crescent were examined to explore the anatomical characteristics of the two types of crescents.

Results: : In B-scan images across the areas of gray crescent, type A gray crescents are seen to represent regions where the retinal pigmented epithelium (RPE) is absent and the dark underlying choroid is directly visible. In contrast, Type B gray crescents represent regions of the retina where the RPE is clumped or thickened, typically appearing to be folded upon itself, revealing the embryonic continuity of the RPE and neuro-retina. Regions that were gray or black on color photographs were bright in the en face SD-OCT images. With Type A crescents, the gray regions on the color photographs are bright in the en face OCT image because of greater illumination of deeper tissue where RPE is absent. In contrast, the Type B crescents are bright in the en face OCT because of the higher reflected signal from the thickened, folded RPE.

Keywords: optic nerve • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • imaging/image analysis: clinical 
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