May 2008
Volume 49, Issue 13
ARVO Annual Meeting Abstract  |   May 2008
Measurement of Various Layers of Huma Retina With Spectral Domain Optical Coherance Tomography in Normal Healthy Volunteers
Author Affiliations & Notes
  • W. W. Phillips
    Ophthalmology, University of Florida, Jacksonville, Florida
  • S. K. Chalam
    Bolles School, Jacksonville, Florida
  • S. Gupta
    Ophthalmology, University of Florida, Jacksonville, Florida
  • Footnotes
    Commercial Relationships  W.W. Phillips, None; S.K. Chalam, None; S. Gupta, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 1882. doi:
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      W. W. Phillips, S. K. Chalam, S. Gupta; Measurement of Various Layers of Huma Retina With Spectral Domain Optical Coherance Tomography in Normal Healthy Volunteers. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1882. doi:

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : A limited number of scans compromises conventional optical coherence tomography (OCT) in estimating retinal thickness accurately. Failures of edge detection algorithms falsify the results of retinal mapping even further. High-definition-OCT (SD-OCT) is based on faster scanning rate (100 times faster) with simultaneous analysis based on spectral characteristics. We investigated and compared the thickeness of retina using both instruments

Methods: : 22 eyes normal healthy volunteers (age ranging from 22 to 66) were imaged using a conventional time domain stratus OCT and high frequency (40khz) spectral-domain, high-speed high resolution Spectralis. Axial resolution was 6 µm; scanning speed was 25 kA-scans/second. Two different scanning patterns covering an area of 6x6mm in the macular retina were compared.

Results: : Detailed information about layer-specific distribution and volumetric measurements were obtained for retinal- and sub-RPE volumes with spectralis. Both raster scans show a high correlation (p<0.01;R2>0.89) of measured values of retinal volume and mean retinal thickness. Retinal thickness could be subdivided into three separte complexes (ganglion cell layer thickness, RPE-IS/OS complex and OS-NFL thickness). True track image technology improved repeatability of measurments (95% repeatability)

Conclusions: : Automatic segmentation allowed for detailed quantitative and topographic analysis of the RPE and the overlying retina. Accurate measurement of various segments of retina is relvevant and useful in a variety of retinal disorders both in diagnosis and in evaluation of theraupetic response to various pharmacological agents.

Keywords: retina: distal (photoreceptors, horizontal cells, bipolar cells) • imaging/image analysis: clinical • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) 

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