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C.-K. Joo, K.-A. Kim, J.-S. Choi; SiRNA of NF-kB Regulates Proliferation and Migration of Lens Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1899. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the role of NF-kappa B and inhibition of lens epithelial cell proliferation in posterior capsular opacity (PCO), this study was performed.
The activation of NF-kappa B was confirmed in cultured human lens epithelial cells (B3 cell lines) and Capsular bag models. The NF-ΚB translocation was confirmed immunohistochemistry and western blot analysis. And sulfasalazine and siRNA were used to inhibit activation of NF-ΚB. The effect of inhibitors was observed by light microscopy and scanning electron microscopy.
The sulfasalazine inhibited translocation of NF-kappa B to nuclear and blocked lens epithelial cell migration in cultured lens epithelial cells and capsular bag model. The lens epithelial cells expressed p105 and p50 of NF-kappa B, normally. The siRNA of NF-kappa B (p105) inhibited the translation of NF-kappa B (p105 and p50). Also, the siRNA inhibits migration of lens epithelial cells.
Suppression of NF-kappa B expression and activation inhibited migration and proliferation in lens epithelial cells. Our result suggests that the regulation of NF-kappa B in lens epithelial cells be able to modulate posterior capsule opacification.
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