May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
SiRNA of NF-kB Regulates Proliferation and Migration of Lens Epithelial Cells
Author Affiliations & Notes
  • C.-K. Joo
    Ophthalmology & Visual Sci, Catholic Univ Korea College of Med, Seoul, Republic of Korea
    Department of Ophthalmology,St. Mary's Hospital, Seoul, Republic of Korea
  • K.-A. Kim
    Ophthalmology & Visual Sci, Catholic Univ Korea College of Med, Seoul, Republic of Korea
  • J.-S. Choi
    Ophthalmology & Visual Sci, Catholic Univ Korea College of Med, Seoul, Republic of Korea
    Korea Eye Tissue and Gene Bank,The Catholic University of Korea, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  C. Joo, None; K. Kim, None; J. Choi, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 1899. doi:https://doi.org/
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      C.-K. Joo, K.-A. Kim, J.-S. Choi; SiRNA of NF-kB Regulates Proliferation and Migration of Lens Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1899. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the role of NF-kappa B and inhibition of lens epithelial cell proliferation in posterior capsular opacity (PCO), this study was performed.

Methods: : The activation of NF-kappa B was confirmed in cultured human lens epithelial cells (B3 cell lines) and Capsular bag models. The NF-ΚB translocation was confirmed immunohistochemistry and western blot analysis. And sulfasalazine and siRNA were used to inhibit activation of NF-ΚB. The effect of inhibitors was observed by light microscopy and scanning electron microscopy.

Results: : The sulfasalazine inhibited translocation of NF-kappa B to nuclear and blocked lens epithelial cell migration in cultured lens epithelial cells and capsular bag model. The lens epithelial cells expressed p105 and p50 of NF-kappa B, normally. The siRNA of NF-kappa B (p105) inhibited the translation of NF-kappa B (p105 and p50). Also, the siRNA inhibits migration of lens epithelial cells.

Conclusions: : Suppression of NF-kappa B expression and activation inhibited migration and proliferation in lens epithelial cells. Our result suggests that the regulation of NF-kappa B in lens epithelial cells be able to modulate posterior capsule opacification.

Keywords: posterior capsular opacification (PCO) • proliferation 
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