May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Corneal Endothelial Cell Damage Due to Air Bubble Trauma in Endothelial Keratoplasty
Author Affiliations & Notes
  • M. Caldwell
    Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • A. N. Kuo
    Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • M. Soper
    North Carolina Eye Bank, Winston-Salem, North Carolina
  • N. A. Afshari
    Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • Footnotes
    Commercial Relationships  M. Caldwell, None; A.N. Kuo, None; M. Soper, None; N.A. Afshari, None.
  • Footnotes
    Support  Research to Prevent Blindness, The North Carolina Eye Bank
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 1942. doi:https://doi.org/
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      M. Caldwell, A. N. Kuo, M. Soper, N. A. Afshari; Corneal Endothelial Cell Damage Due to Air Bubble Trauma in Endothelial Keratoplasty. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1942. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate corneal endothelial cell damage in DSEK graft tissue due to anterior chamber air bubble trauma.

Methods: : Six human research corneas acquired from the North Carolina Eye Bank were sectioned 300 micrometers deep using a Moria mechanical microkeratome and stored in Optisol GS at 4°C. Half of the paired corneas were placed on a Moria artificial chamber and a 30-40% air bubble was injected. The artificial chamber was then turned upside down and back 50 times to simulate normal bubble shifting that occurs in the first 48 hours following DSEK surgery. The corneas were then manually cut with an 8mm trephine and the endothelium was stained with trypan blue 0.25% and alizeran red S 0.2%. The control corneas were similarly cut and stained. All corneas were then photographed at 25x magnification, a montage was created with Photoshop, and the percentage of the total area of damaged endothelium was then calculated for each cornea.

Results: : The mean donor age was 44 years (SD=24). The mean postmortem interval was 13.5 days (SD=10.5) and the mean postcut interval was 39 hours (SD=1.26). The mean corneal endothelial cell density prior to tissue cut for the air bubble eyes was 2656 cells/mm2 (SD=627) and 2493 cells/mm2 (SD=620) for the control eyes. The mean graft thickness for the bubbled eyes was 153 microns (SD=3) and 155 microns (SD=23) for the control. For bubbled eyes, the mean percentage of damaged endothelium was 56% (range 40 to 75%). In the control group, the mean percentage of damaged endothelium was 22% (range 10 to 40%). Between paired air bubble and control eyes, the mean difference in area of undamaged endothelium was 35% (SD=32%).

Conclusions: : A shifting anterior chamber air bubble has the potential to cause corneal endothelial cell damage, as suggested by this model. Clinically, this factor might be considered when determining the size, and therefore duration, of anterior chamber bubbles. Further studies are needed to determine the in-vivo clinical significance.

Keywords: cornea: endothelium • cell survival • transplantation 
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