May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
A New Technique for Corneal Endothelial Graft Insertion With Ciliary Sulcus Fixation Suture in DSEK Cases
Author Affiliations & Notes
  • M. Inoue
    Department of Ophthalmology and Visual Neuroscience, Institute of Health Biosciences,, Tokushima, Japan
  • H. Eguchi
    The University of Tokushima School of Medicine, Tokushima, Japan
  • T. Katome
    The University of Tokushima School of Medicine, Tokushima, Japan
  • S. Matsushita
    The University of Tokushima School of Medicine, Tokushima, Japan
  • Y. Ogi
    The University of Tokushima School of Medicine, Tokushima, Japan
  • H. Shiota
    The University of Tokushima School of Medicine, Tokushima, Japan
  • Footnotes
    Commercial Relationships  M. Inoue, None; H. Eguchi, None; T. Katome, None; S. Matsushita, None; Y. Ogi, None; H. Shiota, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 1944. doi:https://doi.org/
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    • Get Citation

      M. Inoue, H. Eguchi, T. Katome, S. Matsushita, Y. Ogi, H. Shiota; A New Technique for Corneal Endothelial Graft Insertion With Ciliary Sulcus Fixation Suture in DSEK Cases. Invest. Ophthalmol. Vis. Sci. 2008;49(13):1944. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To describe a new surgical technique for corneal endothelial graft insertion into the anterior chamber in DSEK (Descemet’s stripping with endothelial keratoplasty) cases.

Methods: : Three eyes of three cases underwent DSEK using a new technique for endothelial graft insertion with double armed 10-0 prolene that was developed originally for IOL ciliary sulcus fixation (CSF) suture. In all cases, endothelial grafts were prepared by punching pre-cut donor corneas with 7.5 to 9.0 diameter trephine. After holding the endothelial graft over with the endothelial side inward, a stitch was putted on the stroma with a curved needle and another side straight needle was pulled into the anterior chamber. The graft was positioned by pulling the straight needle without any mechanical trauma to the endothelial side and by filling the anterior chamber with air injection. No troubles related with CSF suture occurred during surgeries. In one case that had a penetrating keratoplasty previously, graft reattaching was needed postoperatively because the donor tissue was not well adherent at the host-graft interface. At one year, grafts are well positioned and central endothelial cell losses are less than 20% in another two cases.

Conclusions: : Our new technique for DSEK without any special instrument might be very safe and has minimal damage to endothelial graft.

Keywords: cornea: endothelium • transplantation 
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