May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Contribution of Retinal Ganglion Cells to Intrinsic Signals in Retinal Optical Imaging by Trans-Corneal Electrical Stimulation or by Light Stimulation
Author Affiliations & Notes
  • T. Fujikado
    Osaka Univ Medical School, Suita, Japan
    Dept of Applied Vis Science,
  • Y. Okawa
    Osaka Univ Medical School, Suita, Japan
    Dept of Applied Vis Science,
  • T. Miyoshi
    Osaka Univ Medical School, Suita, Japan
    Dept of Physiology,
  • Y. Hirohara
    Topcon Research Institute, Tokyo, Japan
  • T. Mihashi
    Topcon Research Institute, Tokyo, Japan
  • Y. Tano
    Osaka Univ Medical School, Suita, Japan
    Dept of Ophthalmology,
  • Footnotes
    Commercial Relationships  T. Fujikado, None; Y. Okawa, None; T. Miyoshi, None; Y. Hirohara, Topcon Co., E; T. Mihashi, Topcon Co., E; Y. Tano, None.
  • Footnotes
    Support  MHLWJ Grant 18591918
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2003. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      T. Fujikado, Y. Okawa, T. Miyoshi, Y. Hirohara, T. Mihashi, Y. Tano; Contribution of Retinal Ganglion Cells to Intrinsic Signals in Retinal Optical Imaging by Trans-Corneal Electrical Stimulation or by Light Stimulation. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2003. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Methods:
 

Three eyes of three cats were studied under general anesthesia. For TcES, the electrical current was applied from the fixed frame with rectangular, train of 20 paired biphasic pulses. For LS, 4 cycles/degree black and white squares alternating at 8 Hz were projected as a vertical bar for 4 sec. Fundus images in the near-infrared (800-880nm) were obtained in every 25msec between 2 seconds before and 20 seconds after stimulation. Two-dimensional topography of reflectance change (RC) was constructed by subtracting images before stimulation from those after stimulation. Experiments were carried out before and after TTX (5mM, 20ul) injection intravitreally.

 
Results:
 

RC was observed at OD by TcES (-0.45% at 1.0mA) and both at OD (-0.25%), and light-stimulated retina (LSR, -0.75%) by LS. After TTX application, the RC at OD disappeared almost completely by TcES and LS but those at LSR by LS reduced only 15-20%.

 
Conclusions:
 

The blockage of RGC activity by TTX extinguished the RC at optic disc, suggesting that the intrinsic signal at optic disc may represent the activity of RGCs and could be used to evaluate the RGCs functionally.  

 
Keywords: imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • ganglion cells • optic nerve 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×