May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Alterations in Retinal Blood Flow in the Early Stages of BRAO in Rats
Author Affiliations & Notes
  • S. Tummala
    Biomedical Engineering, Illinois Institute of Technology, Chicago, Illinois
  • S. Benac
    Biomedical Engineering, Illinois Institute of Technology, Chicago, Illinois
  • K. Triandafilou
    Biomedical Engineering, Illinois Institute of Technology, Chicago, Illinois
  • J. J. Kang Derwent
    Biomedical Engineering, Illinois Institute of Technology, Chicago, Illinois
  • Footnotes
    Commercial Relationships  S. Tummala, None; S. Benac, None; K. Triandafilou, None; J.J. Kang Derwent, None.
  • Footnotes
    Support  The Whitaker Foundation
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2085. doi:https://doi.org/
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      S. Tummala, S. Benac, K. Triandafilou, J. J. Kang Derwent; Alterations in Retinal Blood Flow in the Early Stages of BRAO in Rats. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2085. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The objective of this study was to quantify changes in retinal blood flow in vessels neighbouring a branch retinal arterial occlusion (BRAO).

Methods: : All measurements were obtained from anesthetized adult pigmented rats. BRAO was achieved using a photosensitive dye Rose Bengal (20mg/kg) and high intensity xenon white light. Retinal blood velocity was determined by tracking 1µm yellow-green fluorescent polystyrene microspheres (505/515nm maximum absorption/emission) with a Scanning Laser Ophthalmoscope (SLO). Velocity measurements were obtained before, and in 30-minute intervals after induction of occlusion (post-occlusion) and analyzed using particle-tracking velocimetry. Vessel diameters were measured from SLO infrared reflectance (IR) images. Blood flow rate was determined from average velocity and cross-sectional area. Fluorescein angiography was performed to confirm occlusion and determine vessel types. Post-occlusion measurements were acquired for a period of ~3 hours.

Results: : A gradual decrease in velocities in adjacent vessels was observed after induction of branch arterial occlusion. By 2 hours post-occlusion velocities in adjacent arteries (60-80µm diameters) decreased significantly by ~16%, while those in adjacent veins (50-100µm diameter) decreased significantly by ~36%. Diameters of these vessels showed no significant changes over the investigated time period. Arterial and venous blood flow rates decreased significantly by ~21% and ~30% within 2 hours post-occlusion. Systemic blood pressure and other vital signs showed no dramatic changes due to occlusion.

Keywords: vascular occlusion/vascular occlusive disease • blood supply 
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