May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Tauroursodeoxycholic Acid (TUDCA) Slows Retinal Degeneration in Transgenic P23H Rats
Author Affiliations & Notes
  • L. Fernandez-Sanchez
    Fisiologia, Genetica y Microbiologia, Universidad de Alicante, Alicante, Spain
  • I. Pinilla
    Servicio de Oftalmología, Hospital Miguel Servet, Zaragoza, Spain
  • L. Campello
    Fisiologia, Genetica y Microbiologia, Universidad de Alicante, Alicante, Spain
  • M. Idiope
    Servicio de Oftalmología, Hospital Miguel Servet, Zaragoza, Spain
  • J. Martin-Nieto
    Fisiologia, Genetica y Microbiologia, Universidad de Alicante, Alicante, Spain
  • N. Cuenca
    Fisiologia, Genetica y Microbiologia, Universidad de Alicante, Alicante, Spain
  • Footnotes
    Commercial Relationships  L. Fernandez-Sanchez, None; I. Pinilla, None; L. Campello, None; M. Idiope, None; J. Martin-Nieto, None; N. Cuenca, None.
  • Footnotes
    Support  Supported by grants MEC BFU2006-00957/BFI, ONCE and Fundaluce (to N.C.) and Generalitat Valenciana GV06/197 (to J.M–N)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2195. doi:https://doi.org/
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    • Get Citation

      L. Fernandez-Sanchez, I. Pinilla, L. Campello, M. Idiope, J. Martin-Nieto, N. Cuenca; Tauroursodeoxycholic Acid (TUDCA) Slows Retinal Degeneration in Transgenic P23H Rats. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2195. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Systemic injections of TUDCA have been shown to suppress apoptosis and preserve function and morphology of photoreceptor cells in mouse models of retinal degeneration. The purpose of this study was to investigate the possible preventive effect of TUDCA in the rhodopsin P23H transgenic rat model of autosomal dominant retinitis pigmentosa.

Methods: : Homozygous P23H line-3 rats were used in this study. Animals (20 days to 4 months old) were injected TUDCA (500 mg/kg, i.p.) once a week. Cryostat vertical sections of retinas were subjected to single and double immunostaining with antibodies to specific neuronal markers. Images were obtained by means of immunofluorescence confocal microscopy. Retinal function was assessed by electroretinogram. NAPDH-diaphorase histochemistry on whole-mount retinas was performed to visualize the retinal vascular network. Apoptotic cells were identified by using the TUNEL assay.

Results: : Recoverin and transducin immunoreactivity shows a preservation of photoreceptor inner and outer segments present in the retina of TUDCA-treated rats as compared to vehicle-treated subjects. In the latter the number of rows in the ONL was of 2-3 in the nasal area, 3 in the central retina, and 0-1 rows in the temporal retina. However, in TUDCA-treated animals 3-4 rows of photoreceptors were found in the nasal area, 4-6 in central retina and 2-3 in the temporal area. The number of TUNEL-labeled cells was lower in TUDCA-treated animals as compared with controls, indicating a decrease in apoptotic cells. In 4-month old P23H rats the capillary retinal network was significantly reduced with respect to wild-type SD rats. Even, capillary loops were difficult to visualize in the P23H rat. In contrast, the capillary network of TUDCA-treated animals was similar in appearance to that of SD rats, suggesting a positive effect of this compound on the prevention of retinal degeneration.

Conclusions: : TUDCA slows photoreceptor cells degeneration and vascular network disruption in P23H rats. This work indicates that the antiapoptotic action of TUDCA could be potentially useful to retard retinal degeneration in retinitis pigmentosa.

Keywords: neuroprotection • apoptosis/cell death • retinal degenerations: cell biology 
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