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T. Sakai, M. Kondo, K. Komeima, Y. Kurimoto, T. Koyasu, K. Miyata, H. Terasaki; ERG Findings at Early Stages of Retinal Degeneration in Rhodopsin Transgenic Rabbits. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2209. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the properties of the electroretinograms (ERGs) at the early stages of retinal degeneration in a transgenic rabbit with a Pro347Leu rhodopsin mutation.
Full-field ERGs were recorded from five transgenic rabbits with the highest level of expression of the mutant rhodopsin gene at 12-weeks-of-age. Scotopic ERG intensity-response series were recorded after 30 minutes dark-adaptation, and photopic ERGs were recorded after 10 minutes of light-adaptation with a steady background of 1.3 log cd/m2. The amplitudes of the a- and b-waves of the transgenic rabbits were compared to those of five age-matched wild-type rabbits.
The amplitudes for all ERG components were significantly reduced in the transgenic rabbits at 12-weeks-of-age. The amplitudes of the scotopic ERG were more severely reduced than those of the photopic ERGs. With the maximal stimulus intensity of 2.2 log cd-s/m2, the relative amplitude of transgenic rabbits to the mean of wild-type rabbits was 29.4±6.0% for the scotopic a-wave, 37.7±6.5% for the scotopic b-wave, 52.1±14.0% for photopic a-wave, and 74.1±20.2% for photopic b-wave.
These results indicate that there is a predominant rod dysfunction in rhodopsin Pro347Leu transgenic rabbits.
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