May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
ERG Findings at Early Stages of Retinal Degeneration in Rhodopsin Transgenic Rabbits
Author Affiliations & Notes
  • T. Sakai
    Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • M. Kondo
    Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • K. Komeima
    Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • Y. Kurimoto
    Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • T. Koyasu
    Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • K. Miyata
    Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • H. Terasaki
    Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • Footnotes
    Commercial Relationships  T. Sakai, None; M. Kondo, None; K. Komeima, None; Y. Kurimoto, None; T. Koyasu, None; K. Miyata, None; H. Terasaki, None.
  • Footnotes
    Support  Health Sciences Research Grants (H16-sensory-001)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2209. doi:https://doi.org/
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    • Get Citation

      T. Sakai, M. Kondo, K. Komeima, Y. Kurimoto, T. Koyasu, K. Miyata, H. Terasaki; ERG Findings at Early Stages of Retinal Degeneration in Rhodopsin Transgenic Rabbits. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2209. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the properties of the electroretinograms (ERGs) at the early stages of retinal degeneration in a transgenic rabbit with a Pro347Leu rhodopsin mutation.

Methods: : Full-field ERGs were recorded from five transgenic rabbits with the highest level of expression of the mutant rhodopsin gene at 12-weeks-of-age. Scotopic ERG intensity-response series were recorded after 30 minutes dark-adaptation, and photopic ERGs were recorded after 10 minutes of light-adaptation with a steady background of 1.3 log cd/m2. The amplitudes of the a- and b-waves of the transgenic rabbits were compared to those of five age-matched wild-type rabbits.

Results: : The amplitudes for all ERG components were significantly reduced in the transgenic rabbits at 12-weeks-of-age. The amplitudes of the scotopic ERG were more severely reduced than those of the photopic ERGs. With the maximal stimulus intensity of 2.2 log cd-s/m2, the relative amplitude of transgenic rabbits to the mean of wild-type rabbits was 29.4±6.0% for the scotopic a-wave, 37.7±6.5% for the scotopic b-wave, 52.1±14.0% for photopic a-wave, and 74.1±20.2% for photopic b-wave.

Conclusions: : These results indicate that there is a predominant rod dysfunction in rhodopsin Pro347Leu transgenic rabbits.

Keywords: electroretinography: non-clinical • retinal degenerations: hereditary • transgenics/knock-outs 
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