May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Glutathione Redox Balance in Acute Ultraviolet Radiation Cataract
Author Affiliations & Notes
  • S. Lofgren
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • J. Wang
    The Second Affiliated Hospital of Shandong Medical University, Shandong, China
  • P. G. Söderberg
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • Footnotes
    Commercial Relationships  S. Lofgren, None; J. Wang, None; P.G. Söderberg, None.
  • Footnotes
    Support  Karolinska Institutet travel funds. Kronprinsessan Margaretas Fond. Stiftelsen Synfrämjandets forskningsfond.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2270. doi:https://doi.org/
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    • Get Citation

      S. Lofgren, J. Wang, P. G. Söderberg; Glutathione Redox Balance in Acute Ultraviolet Radiation Cataract. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2270. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Solar ultraviolet radiation is the major avoidable risk factor for cataract development. Here we examine short term cataractogenesis and redox balance after a single UVR 300 nm exposure.

Methods: : Sprague-Dawley rats were exposed during 15 minutes to 8 kJ/m2 UVR type B centered at 300 nm. The degree of cataract was quantified by in vitro lens light scattering measurements at 1, 3 and 7 days after UVR exposure. Whole lens reduced and oxidized glutathione and activity of glutathione reductase were measured spectrophotometrically.

Results: : Lens light scattering increased throughout the 7 days following UVR exposure. Exposed lenses exhibited significantly more light scattering than control lenses, at all time points. Reduced glutathione did not change significantly at day 1 but decreased significantly at day 3 and 7. Oxidized glutathione did not change significantly throughout the 7 days. Glutathione reductase activity was significantly increased at day 1 and returned towards baseline at day 3 and 7.

Conclusions: : Acute ultraviolet radiation causes cataract and oxidative changes in parallel. The altered redox balance is not normalized within 7 days after ultraviolet irradiation.

Keywords: cataract • oxidation/oxidative or free radical damage • radiation damage: light/UV 
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