May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Bilateral Cataract Induced by Unilateral UVR-B Exposure- Evidence for an Inflammatory Response
Author Affiliations & Notes
  • L. M. Meyer
    University Eye Clinic Bonn, Bonn, Germany
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • A. Wegener
    University Eye Clinic Bonn, Bonn, Germany
  • S. Löfgren
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • X. Dong
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • V. Mody
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • K. Galichanin
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • F. Holz
    University Eye Clinic Bonn, Bonn, Germany
  • P. Soderberg
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Germany
  • Footnotes
    Commercial Relationships  L.M. Meyer, None; A. Wegener, None; S. Löfgren, None; X. Dong, None; V. Mody, None; K. Galichanin, None; F. Holz, None; P. Soderberg, None.
  • Footnotes
    Support  BONFOR foundation, Bertil Stohnes Stipend
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2271. doi:https://doi.org/
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    • Get Citation

      L. M. Meyer, A. Wegener, S. Löfgren, X. Dong, V. Mody, K. Galichanin, F. Holz, P. Soderberg; Bilateral Cataract Induced by Unilateral UVR-B Exposure- Evidence for an Inflammatory Response. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2271. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate if unilateral in vivo UVR-B exposure of one eye affects the contralateral eye in a co-cataractogenic, sympathetic reaction and to determine if an inflammatory response is involved in the pathogenesis.

Methods: : Eighty C57BL/6 mice were divided into four dose groups (n=20). Depending on dose group animals were unilaterally exposed in vivo to UVR-B for 15 minutes to 0x/ 2x/ 3x/ or 4x cataract threshold dose (MTD2.3:16) UVR-B. The radiation output of the UVR- source had lMAX at 302.6 nm with 4.5 nm [FWHM]. 48 hours following UVR - B exposure cataract was quantified as lens light scattering in the exposed and the contralateral not exposed lenses with a light dissemination meter. Cataract morphology was documented using dark field illumination photography. Serum levels of pro-inflammatory cytokines IL-1ß, IL-6 and TNF-α were measured with ELISA. Immunohistochemistry was performed for neutrophiles and macrophages with antibodies F4/80 and RB6-8C5 in exposed and contralateral eyes.

Results: : Exposed lenses scattered light higher than non-exposed lenses. The difference was significant at 2 & 4 MTD2.3:16 exposure dose. Mean light scattering in contralateral lenses in 2/ 3/ 4 MTD group was increased as compared to the control group(95% CI for mean light scattering in contralateral eyes:0 MTD= 0.227+/- 0.02 tEDC; 2 MTD= 0.261 +/- 0.02 tEDC; 3 MTD = 0.303 +/- 0.04 tEDC; 4 MTD= 0.319 +/- 0.04 tEDC). Serum pro-inflammatory cytokine levels were increased for IL-1β, IL-6 but not for TNF-α Inflammatory infiltration was detected with immunohistochemistry in the anterior segment of exposed eyes as well as in some mice also in the non-exposed eye.

Conclusions: : Unilateral UVR-B exposure increases light scattering also in the contralateral eye and triggers a systemic inflammatory response mediated by IL-1β and IL-6. Age related cataract is almost exclusively a bilateral event. Since a systemic response might be an important factor in the pathogenesis of the disease our results might initiate new approaches in cataract research.

Keywords: cataract • oxidation/oxidative or free radical damage • inflammation 
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