May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Anterior Lens Capsule Thickness in Diabetic and Non-Diabetic Dogs
Author Affiliations & Notes
  • L. M. Barros
    Surgery, University of Sao Paulo, Sao Paulo, Brazil
  • B. F. Fernandes
    Ophthalmology, McGill University, Montreal, Quebec, Canada
  • S. Bakalian
    Ophthalmology, McGill University, Montreal, Quebec, Canada
  • A. M. V. Safatle
    Surgery, University of Sao Paulo, Sao Paulo, Brazil
  • P. S. M. Barros
    Surgery, University of Sao Paulo, Sao Paulo, Brazil
  • M. N. Burnier, Jr.
    Ophthalmology, McGill University, Montreal, Quebec, Canada
  • Footnotes
    Commercial Relationships  L.M. Barros, None; B.F. Fernandes, None; S. Bakalian, None; A.M.V. Safatle, None; P.S.M. Barros, None; M.N. Burnier, None.
  • Footnotes
    Support  CAPES fellowship (Barros LM)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2282. doi:
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      L. M. Barros, B. F. Fernandes, S. Bakalian, A. M. V. Safatle, P. S. M. Barros, M. N. Burnier, Jr.; Anterior Lens Capsule Thickness in Diabetic and Non-Diabetic Dogs. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2282.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : to determine if the Anterior Capsule Basement Membrane (ACBM) thickness increases in diabetic dogs (DD) when compared with non diabetic dogs (NDD), and correlate the findings with the duration of diabetes.

Methods: : In this prospective study, anterior lens capsule specimens were collected from routine phaco-emulsification or conventional extra-capsular lens extraction performed by the same surgeon. Thirty eight (38) anterior lens capsule specimens of diabetic dogs aging 8.63±2.28 (mean± SD) years and twenty two (22) of non- diabetic dogs aging 8.42± 2.19 (mean± SD) were formalin-fixed (10% formalin) and paraffin-embedded. Paraffin sections were cut at 5µm, mounted on commercially provided silanized slides dried overnight at 37°C and stored at 60°C for at least 60 minutes. The prepared slides were stained with a standard staining procedure of H&E and PAS using ready-to-use solutions for visual assessment and thickness measurement. The ACBM thickness was measured in units (u) using a Carl-Zeis® 444034 eyepiece. Statistical analysis was performed using Student’s t test to compare ACBM thickness between DD and NDD groups. Regression analysis and Pearson’s correlation were used to assess the relationship between the increase of ACBM thickness in DD and the duration of diabetes.

Results: : The mean thickness of ACBM was 14.41±2.24u (mean± SD) for DD and 12.50±2.98u (mean± SD) for NDD. There was a statistically significant difference when the ACBM thickness of the DD group is compared with the NDD group (p=0.01). There was no correlation between the increase of ACBM thickness in DD and the duration of diabetes (Rsq= 0,003).

Conclusions: : Previous studies have measured the thickness of the anterior lens capsule in dogs, but the effects of diabetes had never been investigated. To the best of our knowledge this is the first study that measures ACMB thickness in DD comparing with NDD. Anterior capsule basement membrane in DD was significantly thicker compared to NDD, confirming that diabetes causes ACBM thickening in DD, however our results showed that the ACMB thickness is not influenced by the duration of diabetes.

Keywords: crystallins • diabetes • cataract 
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