May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Comparative Analysis of Plasma and Lens Gamma-Glutamyltranspeptidase (GGT) Activity in Type 1 and Type 2 Diabetic Rat Models
Author Affiliations & Notes
  • M. P. Langford
    Lousiana State University Health Science Center, Shreveport, Louisiana
    Ophthalmology,
  • K. E. Taba
    Lousiana State University Health Science Center, Shreveport, Louisiana
    Ophthalmology,
  • J. L. Croad
    Lousiana State University Health Science Center, Shreveport, Louisiana
    Pediatrics,
  • J. L. Rains
    Lousiana State University Health Science Center, Shreveport, Louisiana
    Pediatrics,
  • S. K. Jain
    Lousiana State University Health Science Center, Shreveport, Louisiana
    Pediatrics,
  • Footnotes
    Commercial Relationships  M.P. Langford, None; K.E. Taba, None; J.L. Croad, None; J.L. Rains, None; S.K. Jain, None.
  • Footnotes
    Support  NIH Grants DK064797; DK072433
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2286. doi:https://doi.org/
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      M. P. Langford, K. E. Taba, J. L. Croad, J. L. Rains, S. K. Jain; Comparative Analysis of Plasma and Lens Gamma-Glutamyltranspeptidase (GGT) Activity in Type 1 and Type 2 Diabetic Rat Models. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2286. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Elevated GGT is a risk factor for insulin resistance as well as cataract formation. We investigated the hypothesis that a co-ordinate lenticular GGT and glutathione (GSH) response occurs with development of experimental type 1 (T1) and type 2 (T2) diabetes.

Methods: : T1 diabetes was induced by Streptozocin (STZ) treatment in Sprague-Dawley rats and T2 diabetes developed by 16 weeks in Zucker diabetic fatty (ZDF) rats. The pre- and post diabetic plasma and lens levels of GGT and GSH were compared. Plasma samples and whole lenses were obtained from 8-10 rats prior to and 6 to 8 weeks post diabetes detection. The Accu-Chek® plasma glucose concentration, plasma and lens GGT and GSH levels were determined by standard colorimetric assays.

Results: : The GGT activity in the T1 diabetic rat plasma was 5-fold higher (p=0.002) than in control non-diabetic plasma. There was a close association between elevated plasma glucose and increased GGT (r = .0615; slope= 0.534) in STZ diabetic rats. The GGT and GSH levels in T1 diabetic lens were significantly lower (p’s<0.01) than in control non-diabetic lens. The GGT activity in plasma of control ZDF rats was 2-fold lower than in diabetic STZ rats. T2 plasma GGT levels tended to be inversely related to the plasma glucose concentration (r =.0215; slope= -.051). The levels of GGT and GSH in lens were lower in diabetic versus non-diabetic ZDF rats.

Conclusions: : The results suggest that the T1 and T2 diabetic rat models are dissimilar with regard to the relationships between elevated plasma glucose and GGT levels. The results suggest that a co-ordinate reduction in GGT and GSH levels occurs in diabetic rat lens.

Keywords: diabetes • cataract • pathology: experimental 
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