May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Optimization of the Pig Model for Penetrating Keratoplasty
Author Affiliations & Notes
  • T. Bensaoula
    Maisonneuve-Rosemont Hospital Research Center, Montreal, Quebec, Canada
  • O. Nada
    Maisonneuve-Rosemont Hospital Research Center, Montreal, Quebec, Canada
  • S. Proulx
    Departments of Ophthalmology and Surgery / LOEX / Laval University, Quebec, Quebec, Canada
  • L. Germain
    Departments of Ophthalmology and Surgery / LOEX / Laval University, Quebec, Quebec, Canada
  • V. Bernier
    Pathology, Hotel Dieu du CHUQ, Quebec, Quebec, Canada
  • M. Carrier
    Veterinary Medicine,
    University of Montreal, Montreal, Quebec, Canada
  • S. G. Rosolen
    INSERM UMR-592, UPMC-Paris 6 / Fondation Ophtalmologique A. de Rothschild / Institut de la Vision, Paris, France
  • I. Brunette
    Maisonneuve-Rosemont Hospital Research Center, Montreal, Quebec, Canada
    Ophthalmology,
    University of Montreal, Montreal, Quebec, Canada
  • Footnotes
    Commercial Relationships  T. Bensaoula, None; O. Nada, None; S. Proulx, None; L. Germain, None; V. Bernier, None; M. Carrier, None; S.G. Rosolen, None; I. Brunette, None.
  • Footnotes
    Support  CIHR, Ottawa, ON, Canada; FRSQ Research in Vision Network, Montreal, QC, Canad; FROUM, University of Montreal, Montreal, QC, Canada.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2328. doi:https://doi.org/
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      T. Bensaoula, O. Nada, S. Proulx, L. Germain, V. Bernier, M. Carrier, S. G. Rosolen, I. Brunette; Optimization of the Pig Model for Penetrating Keratoplasty. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2328. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The goal of this study was to report on the advantages and limitations of the pig model for experimental in vivo penetrating keratoplasty (PK), and to optimize this model.

Methods: : Eleven healthy domestic pigs aged from 10 to 20 weeks (range: 6 to 120 kg) were used. Eight animals underwent full thickness PK with either allogeneic (n=3) or autologous (n=5) donor corneal tissue. In three cases, the inflammatory response to partial steps of the surgery was evaluated. Animals were observed daily (slit-lamp, tonometry, and pachymetry) and euthanized on day 7. Postmortem assessment included optical coherence tomography, histology and electron microscopy (SEM and TEM).

Results: : Graft failure was observed in 1 case and was attributed to surgically induced intraocular inflammation. Corneal edema was present at the end of surgery in all cases and decreased after surgery (r=-0.87; p=0.002). Inflammation, which was found to be a key parameter for the success of the graft, was significantly less when the following were used at the time of surgery: full pupil dilatation, dispersive viscoelastic agents, intraocular tissue plasminogen activators, intravenous mannitol, and systemic and topical steroids. Age was also a significant parameter for the success of transplantation. Histopathology of successful grafts showed a nice endothelial monolayer, with no adjacent fibrin membrane, while in the failed graft only a few residual endothelial cells remained, covered with a thick inflammatory membrane. SEM of successful grafts confirmed the presence of the typical hexagonal corneal endothelial pattern and TEM showed a normal endothelial cell ultrastructure.

Conclusions: : Adequate surgical and general anesthesia protocols were successfully developed for experimental in vivo PK in the pig model.

Keywords: cornea: clinical science • cornea: basic science • comparative anatomy 
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