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A. A. Torres, T. L. Purcell, A. Ortiz, D. J. Schanzlin; Alternative Device for Graft Insertion in Descemet's Stripping Automated Endothelial Keratoplasty. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2344.
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To determine the feasibility of a new insertion system that is able to atraumatically insert an 8.5 mm stromal endothelial graft into the anterior chamber through a 3 mm incision.
A new fabricated device was designed to insert an 8.5 mm stromal endothelial graft through a 3 mm incision. This device was used in combination with a suture pull through technique without folding the graft or touching the corneal endothelium. This device was tested using 3 human donor corneas and 3 whole cadaveric eyes that were not suitable for transplantation. Following this procedure, the graft tissue was stained with Trypan blue to indicate endothelial damage.
The insertion device allowed us to successfully insert an 8.5 mm stromal endothelial graft into the anterior chamber of the cadaveric human eyes through a 3 mm incision. All 3 grafts indicated minimal to no Trypan blue staining of the endothelium following this procedure.
A new fabricated device is practical for atraumatic insertion of stromal endothelial grafts into the anterior chamber for Descemet’s Stripping Automated Endothelial Keratoplasty.
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