May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Alternative Device for Graft Insertion in Descemet's Stripping Automated Endothelial Keratoplasty
Author Affiliations & Notes
  • A. A. Torres
    Cornea and Refractive Surgery, UCSD Shiley Eye Center, San Diego, California
  • T. L. Purcell
    Cornea and Refractive Surgery, UCSD Shiley Eye Center, San Diego, California
  • A. Ortiz
    Cornea and Refractive Surgery, UCSD Shiley Eye Center, San Diego, California
  • D. J. Schanzlin
    Cornea and Refractive Surgery, UCSD Shiley Eye Center, San Diego, California
  • Footnotes
    Commercial Relationships  A.A. Torres, None; T.L. Purcell, None; A. Ortiz, None; D.J. Schanzlin, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2344. doi:https://doi.org/
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    • Get Citation

      A. A. Torres, T. L. Purcell, A. Ortiz, D. J. Schanzlin; Alternative Device for Graft Insertion in Descemet's Stripping Automated Endothelial Keratoplasty. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2344. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To determine the feasibility of a new insertion system that is able to atraumatically insert an 8.5 mm stromal endothelial graft into the anterior chamber through a 3 mm incision.

Methods: : A new fabricated device was designed to insert an 8.5 mm stromal endothelial graft through a 3 mm incision. This device was used in combination with a suture pull through technique without folding the graft or touching the corneal endothelium. This device was tested using 3 human donor corneas and 3 whole cadaveric eyes that were not suitable for transplantation. Following this procedure, the graft tissue was stained with Trypan blue to indicate endothelial damage.

Results: : The insertion device allowed us to successfully insert an 8.5 mm stromal endothelial graft into the anterior chamber of the cadaveric human eyes through a 3 mm incision. All 3 grafts indicated minimal to no Trypan blue staining of the endothelium following this procedure.

Conclusions: : A new fabricated device is practical for atraumatic insertion of stromal endothelial grafts into the anterior chamber for Descemet’s Stripping Automated Endothelial Keratoplasty.

Keywords: transplantation • cornea: endothelium 
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