May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Subepithelial Corneal Fibrosis Partially Due to Epithelial-Mesenchymal Transition of Limbal Epithelium
Author Affiliations & Notes
  • M. Kawashima
    Department of Ophthalmology, Keio University school of medicine, Shinjuku ku, Japan
  • T. Kawakita
    Department of Ophthalmology, Keio University school of medicine, Shinjuku ku, Japan
  • S. Shimmura
    Department of Ophthalmology, Keio University school of medicine, Shinjuku ku, Japan
  • K. Higa
    Department of Ophthalmology, Tokyo Dental College, Chiba, Japan
  • Y. Satake
    Department of Ophthalmology, Tokyo Dental College, Chiba, Japan
  • J. Shimazaki
    Department of Ophthalmology, Tokyo Dental College, Chiba, Japan
  • K. Tsubota
    Department of Ophthalmology, Keio University school of medicine, Shinjuku ku, Japan
  • Footnotes
    Commercial Relationships  M. Kawashima, None; T. Kawakita, None; S. Shimmura, None; K. Higa, None; Y. Satake, None; J. Shimazaki, None; K. Tsubota, None.
  • Footnotes
    Support  Grant-in-Aid for Young Scientists (B) (18790131),Grant-in-Aid for Scientific Research (H18-tissue engineering-young -002)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2351. doi:https://doi.org/
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    • Get Citation

      M. Kawashima, T. Kawakita, S. Shimmura, K. Higa, Y. Satake, J. Shimazaki, K. Tsubota; Subepithelial Corneal Fibrosis Partially Due to Epithelial-Mesenchymal Transition of Limbal Epithelium. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2351. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To determine whether epithelial-mesenchymal transition (EMT) is involved in corneal subepithelial fibrosis (pannus) in tissue surgically removed from patients with total limbal stem cell deficiency.

Methods: : Frozen sections of pannus tissue removed from 10 human corneas with total limbal stem cell deficiency was examined for their phenotype using anti-keratin (K)-3, K10, K12, K13,15 and K19 monoclonal antibodies.β-catenin and E-cadherin expression were also examined to observe Wnt-pathway activation. p63 and pancytokeratin were used as epithelial markers, and S100A4 andα-smooth muscle actin (α-SMA ) for mesenchymal marker. Epithelium was also removed from pannus after dispase digestion, and cultured for RT-PCR analysis for quantitative analysis.

Results: : Epithelial basal cells in the pannus showed strong nuclear expression of p63, weak E-cadherin, and translocation of beta-catenin from intercellular junctions to the cytoplasm was observed. Furthermore, p63 and S100A4 positive cells were found in the stroma of the pannus.

Conclusions: : Epithelial-mesenchymal transition was partially involved in subepithelial corneal fibrosis due to the total limbal stem cell deficiency.

Keywords: EMT (epithelial mesenchymal transition) • cornea: epithelium • cornea: basic science 
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