May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Corneal Epithelial Disorder Associated With The Antineoplatic Drug S-1
Author Affiliations & Notes
  • T.-I. Chikama
    Ocular Pathophysiology, Yamaguchi University School of Medicine, Ube, Yamaguchi, Japan
  • N. Takahashi
    Dept. Ophthalmology, Toyota Central Hospital, Shimonoseki, Yamaguchi, Japan
  • M. Wakuta
    Ophthalmology, Ube Industries Central Hospital, Ube, Yamaguchi, Japan
  • N. Nomi
    Ophthalmology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan
  • Y. Ishida
    Ophthalmology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan
  • R. Yanai
    Ophthalmology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan
  • T. Nishida
    Ophthalmology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan
  • T. Adachi
    Dept. Ophthalmology, Ogori Daiichi General Hospital, Yamaguchi, Yamaguchi, Japan
  • M. Enoki
    Dept. Ophthalmology, Ogori Daiichi General Hospital, Yamaguchi, Yamaguchi, Japan
  • Footnotes
    Commercial Relationships  T. Chikama, None; N. Takahashi, None; M. Wakuta, None; N. Nomi, None; Y. Ishida, None; R. Yanai, None; T. Nishida, None; T. Adachi, None; M. Enoki, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2354. doi:https://doi.org/
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      T.-I. Chikama, N. Takahashi, M. Wakuta, N. Nomi, Y. Ishida, R. Yanai, T. Nishida, T. Adachi, M. Enoki; Corneal Epithelial Disorder Associated With The Antineoplatic Drug S-1. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2354. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : S-1 is an oral antineoplastic drug that contains tegafur and has been shown to induce corneal disorders. To characterize the cellular changes in the corneal epithelium associated with S-1-induced keratopathy, we examined affected corneal epithelial cells by in vivo confocal microscopy and histopathologic analysis.

Methods: : Three men and three women treated with S-1 participated in the study. The 12 eyes of the six subjects were evaluated with a confocal laser-scanning microscope, consisting of a Heidelberg Retina Tomograph II® attached to a Rostock Cornea Module ®(HRTII-RCM). Corneal epithelial debridement and histopathologic analysis of the specimens were performed for the five eyes of three subjects that were affected in the pupillary zone of the cornea.

Results: : Slitlamp examination revealed a local limbal abnormality characterized by abnormal epithelial invasion toward the central cornea in all 12 eyes. HRTII-RCM examination revealed an altered structure of the corneal epithelium with abnormal epithelial cells. Hematoxylin-eosin staining showed that each abnormal epithelial sheet lacked the stratified structure of the normal corneal epithelium. Immunofluorescence analysis of cytokeratins 12 and 4 revealed the presence of four types of cells-those positive for one, both, or neither of these proteins-in each lesion.

Conclusions: : Four different phenotypes of cells were found in the corneal epithelium affected by S-1 administration. Cells lacking both cytokeratins 12 and 4 are likely undifferentiated cells, whereas those expressing cytokeratin 4 alone are likely conjunctival epithelial cells that entered the cornea through the limbus.

Keywords: cornea: clinical science • drug toxicity/drug effects • imaging/image analysis: clinical 
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