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M. Takaoka, T. Nakamura, H. Sugai, A. J. Bentlay, N. Nakajima, N. J. Fullwood, N. Yokoi, S.-H. Hyon, S. Kinoshita; A Novel Sutureless Amniotic Membrane Transplantation for Ocular Surface Reconstruction with Chemically Defined Bioadhesives. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2355.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the efficiency and safety of a novel sutureless amniotic membrane (AM) transplantation for ocular surface reconstruction with a chemically-defined bioadhesive.
The chemically defined bioadhesive was synthesized from aldehyded polysaccharides and ε-poly(L-lysine), two kinds of antibacterial food additives. To ensure its safety and biocompatibility, rabbit tissues were histologically examined 4 weeks after subconjunctival injection. Sutureless amniotic membrane transplantation (AMT) was performed onto a rabbit scleral surface using the bioadhesive. The interface between the AM and sclera was ultrastructurally examined on 1 day sample. Degrees of hyperemia and epithelialization were evaluated by slit lamp microscopy at 2, 4, 8, and 12 weeks after AMT. To examine its biodegradability to the ocular surface, 4- and 12-week samples were histologically examined. Conjunctiva on the AM was evaluated by electron microscopic- and immunohistochemical examination for its morphological and biological properties.
The chemically defined bioadhesive was histologically degraded within 4 weeks after subconjunctival injection. Sutureless AMT was performed successfully without loss or dislocation. Epithelialization on the AM was achieved within 2 weeks. No additional hyperemia and scarring was observed in the eyes with sutureless AMT compared to those with conventional AMT. Electron microscopy revealed that the adhesive was sufficiently laid between the chorionic side of the AM and the sclera. Electron microscopy and immunohistochemistry showed that the conjunctival epithelium was well stratified without derangement of the biological properties, suggesting that this bioadhesive supports normal cell differentiation.
This chemically defined bioadhesive was found to be very useful for sutureless AMT for ocular surface reconstruction, without needing to consider the risk of infection. It has the ability to fix AM to the ocular surface for a long time without additional inflammation, scarring, or damage to the surrounding tissues.
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