May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Matrix Metalloproteinase Expression and the Effect of Ultraviolet Radiation Exposure on Ocular Surface Squamous Neoplasia
Author Affiliations & Notes
  • N. Di Girolamo
    Sch of Med Sci - Pathology Dept, Univ of New South Wales, Sydney, Australia
  • J. Ng
    Sch of Med Sci - Pathology Dept, Univ of New South Wales, Sydney, Australia
  • M. T. Coroneo
    Dept of Ophthalmology, Prince of Wales Hospital, Sydney, Australia
  • D. Wakefield
    Sch of Med Sci - Pathology Dept, Univ of New South Wales, Sydney, Australia
  • Footnotes
    Commercial Relationships  N. Di Girolamo, None; J. Ng, None; M.T. Coroneo, None; D. Wakefield, None.
  • Footnotes
    Support  NHMRC 455358, ORIA
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2358. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      N. Di Girolamo, J. Ng, M. T. Coroneo, D. Wakefield; Matrix Metalloproteinase Expression and the Effect of Ultraviolet Radiation Exposure on Ocular Surface Squamous Neoplasia. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2358. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Ocular surface squamous neoplasia (OSSN) is an uncommon tumour of the corneal/conjunctival epithelium that is thought to develop as a consequence of excessive ultraviolet (UV) radiation exposure. To date, few studies have been conducted on the molecular mechanisms of this disease. The purpose of this study was (i) to identify and localize potential mediators responsible for the development of this disease and (ii)to culture human dysplastic epithelial cells (DEC) and determine their responsiveness to UVB radiation compared to normal epithelial cells (CEC).

Methods: : Immunohistochemical analysis was performed on formalin-fixed paraffin-embedded OSSN (n=23) and normal conjunctival (n=17) tissue with antibodies to MMPs and TIMPs. Basal as well as UVB-modulated levels of MMPs and TIMPs from DEC and CEC were determined by immunoassay, zymography and RT-PCR.

Results: : A higher proportion of diseased specimens stained for MMP-1 (83%), MMP-3 (86%), TIMP-2 (87%), and TIMP-3 (83%) compared to normal conjunctiva (41%, 41%, 47%, 53% respectively). Although TIMP-1 expression seemed more abundant in normal conjunctiva (76%) compared to diseased (61%) tissue, this did not reach statistical significance. MMP-2 was evenly distributed in both groups. UVB radiation induced cell death and apoptosis at doses greater than 50 mJ/cm2 compared to 20 mJ/cm2 which caused no significant apoptosis or morphological change. Interestingly, in DECs, mRNA and protein for MMP-1 and MMP-3 were dose-dependently induced following irradiation, while the same enzymes were only up-regulated in normal CEC at doses that induced apoptosis. TIMP-1 and -2 levels remained relatively unchanged, except for a dose-dependent suppression of TIMP-3.

Conclusions: : MMPs and TIMPs may play a significant role in the pathogenesis of OSSN and that UVB may perpetuate the development of this lesion on the ocular surface. These findings suggest UVB avoidance is prudent, both in patients with OSSN, and in the healthy population.

Keywords: cornea: epithelium • radiation damage: light/UV • tumors 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×