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W. Wee, K. Chang, M. Kim, J.-W. Ko, H.-J. Lee, J. Lee, M. Park; The Effect of Uridine on the Cells of Conjunctiva and Cornea in vitro and in vivo Model. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2370.
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To evaluate the effect of uridine on the migration of corneal epithelial cells and to investigate the effect of oral uridine on ocular surface in rabbit dry eye model.
Migration studies were performed using confluent monolayers of cultured human corneal epithelial cell. Uridine and UTP (1,10,100 µM, respectively) were applied with or without P2Y antagonist (PPADS, 100 µM) after cell sheets were wounded with a pipette tip. Concanavalin A(Con A) was injected into both lacrimal glands twice at three-day intervals in 20 eyes of 10 rabbits. From 3 days after injection, oral uridne group(n=5) was given uridine (84mg/kg) mixed with water (400ml) orally and control group(n=5) was taken only water (400ml) for 7 days. Schirmer I test and Impression cytology were checked on 3 days and 10 days after Con A injection. The changes of tear secretion and goblet cell density were compared between before- and after-treatment in each group or between those two groups.
It showed that Uridine accelerated the cell migrations as did UTP and it’s effect was antagonized by PPADS. In contrast to control group, goblet cell density in oral urdine group was not significantly decreased and it showed significant difference between those two groups (P=0.043 Mann-Whitney U test). There was no significant change of tear secretion in both groups.
Application of uridine may be beneficial for corneal wound healing including dry eye.
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