May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
TACE(ADAM17) Dependent Shedding of Soluble TNF Receptor in Conjunctival Epithelium
Author Affiliations & Notes
  • T. Sakimoto
    Dept of Ophthalmology, Nihon Univ School of Medicine, Itabashi-ku, Japan
  • A. Yamada
    Dept of Ophthalmology, Nihon Univ School of Medicine, Itabashi-ku, Japan
  • J. Shoji
    Dept of Ophthalmology, Nihon Univ School of Medicine, Itabashi-ku, Japan
  • M. Sawa
    Dept of Ophthalmology, Nihon Univ School of Medicine, Itabashi-ku, Japan
  • Footnotes
    Commercial Relationships  T. Sakimoto, None; A. Yamada, None; J. Shoji, None; M. Sawa, None.
  • Footnotes
    Support  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C) 18591937
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2393. doi:https://doi.org/
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    • Get Citation

      T. Sakimoto, A. Yamada, J. Shoji, M. Sawa; TACE(ADAM17) Dependent Shedding of Soluble TNF Receptor in Conjunctival Epithelium. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2393. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate the involvement of tumor necrosis factor-alpha converting enzyme (TACE, ADAM17) in ectodomain shedding of TNF receptor in conjunctival epithelium.

Methods: : The corneal alkali burn of BALB/c mice was made using a filter paper dipped in 1N NaOH solution. TNF receptor 1 (TNFR1) expression in conjunctiva was evaluated by immunohistochemistry with time course (3eyes each). In vitro experiments were performed using human conjunctival epithelial cell (HCjEC) line. Peptidoglycan (PGN, 100µg/mL, 24hours) alone or PGN and TACE inhibitor (TAPI-1, 250µg/mL, 4hours) were added to HCjEC medium. s (soluble) TNFR1 concentration in supernatant of the culture medium was analyzed by ELISA.

Results: : In corneal alkali burn model, TNFR1 was expressed in conjunctival epithelium 14 days after the alkali burn. In HCjEC cultured medium, sTNFR1 release was significantly increased by adding of PGN (t-test, p<0.05). This increased release of sTNFR1 was significantly inhibited by TAPI-1 (t-test, p<0.05), indicating the participation of TACE in this sTNFR1 releasing process.

Conclusions: : In conjunctiva, TNFR1 was expressed in the epithelium after the corneal alkali burn and TACE dependent shedding of sTNFR1 was recognized in conjunctival epithelium. sTNFR1, released from ocular surface, might play anti-inflammatory role in inflammatory condition by neutralizing TNF-α.

Keywords: inflammation • conjunctiva • cytokines/chemokines 
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