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D. A. Dartt, L. Jonsson, R. R. Hodges, L. M. Tarko, C. W. Zeldis, M. A. Shatos; Nestin-Positive Myoepithelial Cells Are Progenitor Cells in the Lacrimal Gland. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2451. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To identify progenitor cells in the lacrimal gland.
Rat lacrimal glands were dissociated by repetitive cycles of digestion in Type I collagenase. Liberated cells were first grown in serum-supplemented RPMI-1640 medium. To examine plasticity of precursor cells, they were cultured in either serum-free RPMI medium or in X-Vivo medium containing various growth factors. Characterization medium consisted of either RPMI or X-Vivo medium supplemented with 10% FBS. Cells were evaluated for expression of the progenitor cell marker, nestin; the proliferation markerKi-67; the myoepithelial cell markers smooth muscle actin, α-actinin, vimentin, and adenyl cyclase II; the neuronal markers NF-200 and MAP 5; and the glial cell marker, GFAP. Tissue sections of lacrimal gland were also examined for the presence of nestin.
Following digestion with collagenase, a variety of cells immediately attached to the culture vessel and became confluent. Gradually, clusters of round immature cells that were nestin and Ki-67 positive began to form and overgrew the underlying monolayer. In FBS-supplemented RPMI medium these cells differentiated into myoepithelial cells expressing smooth muscle actin, α-actinin, vimentin and adenyl cyclase II. When tissue sections of lacrimal gland were evaluated for nestin expression, nestin was observed in a population of myoepithelial cells surrounding both acini and ducts. In separate experiments to examine plasticity of these immature precursor cells, they were grown in X-Vivo neural cell medium that caused them to differentiate into neuronal-like cells, which expressed NF-200 and MAP-5, but not the glial cell marker GFAP.
Lacrimal gland myoepithelial cells appear to originate from progenitor cells within the gland and may retain some progenitor cell functions.
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