May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Nestin-Positive Myoepithelial Cells Are Progenitor Cells in the Lacrimal Gland
Author Affiliations & Notes
  • D. A. Dartt
    Schepens Eye Research Institute, Boston, Massachusetts
  • L. Jonsson
    Schepens Eye Research Institute, Boston, Massachusetts
  • R. R. Hodges
    Schepens Eye Research Institute, Boston, Massachusetts
  • L. M. Tarko
    Schepens Eye Research Institute, Boston, Massachusetts
  • C. W. Zeldis
    Schepens Eye Research Institute, Boston, Massachusetts
  • M. A. Shatos
    Schepens Eye Research Institute, Boston, Massachusetts
  • Footnotes
    Commercial Relationships  D.A. Dartt, None; L. Jonsson, None; R.R. Hodges, None; L.M. Tarko, None; C.W. Zeldis, None; M.A. Shatos, None.
  • Footnotes
    Support  NIH grant EY06177
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2451. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      D. A. Dartt, L. Jonsson, R. R. Hodges, L. M. Tarko, C. W. Zeldis, M. A. Shatos; Nestin-Positive Myoepithelial Cells Are Progenitor Cells in the Lacrimal Gland. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2451. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To identify progenitor cells in the lacrimal gland.

Methods: : Rat lacrimal glands were dissociated by repetitive cycles of digestion in Type I collagenase. Liberated cells were first grown in serum-supplemented RPMI-1640 medium. To examine plasticity of precursor cells, they were cultured in either serum-free RPMI medium or in X-Vivo medium containing various growth factors. Characterization medium consisted of either RPMI or X-Vivo medium supplemented with 10% FBS. Cells were evaluated for expression of the progenitor cell marker, nestin; the proliferation markerKi-67; the myoepithelial cell markers smooth muscle actin, α-actinin, vimentin, and adenyl cyclase II; the neuronal markers NF-200 and MAP 5; and the glial cell marker, GFAP. Tissue sections of lacrimal gland were also examined for the presence of nestin.

Results: : Following digestion with collagenase, a variety of cells immediately attached to the culture vessel and became confluent. Gradually, clusters of round immature cells that were nestin and Ki-67 positive began to form and overgrew the underlying monolayer. In FBS-supplemented RPMI medium these cells differentiated into myoepithelial cells expressing smooth muscle actin, α-actinin, vimentin and adenyl cyclase II. When tissue sections of lacrimal gland were evaluated for nestin expression, nestin was observed in a population of myoepithelial cells surrounding both acini and ducts. In separate experiments to examine plasticity of these immature precursor cells, they were grown in X-Vivo neural cell medium that caused them to differentiate into neuronal-like cells, which expressed NF-200 and MAP-5, but not the glial cell marker GFAP.

Conclusions: : Lacrimal gland myoepithelial cells appear to originate from progenitor cells within the gland and may retain some progenitor cell functions.

Keywords: lacrimal gland • cornea: tears/tear film/dry eye 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×