Purchase this article with an account.
E. C. Ledbetter, E. J. Dubovi, S. G. Kim, D. J. Maggs; Experimental Canine Herpesvirus-1 Primary Ocular Infection in Adult Dogs. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2485. doi: https://doi.org/.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To establish the ocular pathogenic potential of canine herpesvirus-1 (CHV-1) in adult dogs and to characterize clinical ocular disease, viral shedding, and serologic response of primary CHV-1 ocular infection.
Eight 1.5 year old specific pathogen-free (CHV-1 free) beagles were inoculated in the right eye with 2x105 TCIM50 of CHV-1 by corneal microtrephination (n = 4 dogs) or conjunctival drop (n = 4) methods. Forty-eight hours prior to inoculation, 4 of the dogs (2 from each inoculation method group) received 10 mg of methylprednisolone acetate subconjunctivally in the right eye. Following inoculation, physical and slit-lamp biomicroscopic examinations were performed daily for the first 21 days post-infection (PI) and every-other-day for the following 14 days. Conjunctival swabs for real-time quantitative CHV-1 PCR and virus isolation were collected from both eyes for 35 days PI. Buffy coats were collected for CHV-1 PCR for 35 days PI. Serum for CHV-1 serum neutralization (SN) titers was collected for 119 days PI.
All dogs developed bilateral, mild-to-moderate conjunctivitis that reached maximal intensity 7-10 days PI and resolved over the subsequent 21 days. Ocular viral shedding was present from the right eye of all dogs and the left eye of 2 dogs between 3 and 10 days PI. All dogs developed CHV-1 SN titers, beginning on day 7 PI, peaking on day 21 PI, and declining over the subsequent weeks. All buffy coat CHV-1 PCR assays were negative. No dogs developed clinical systemic disease. Following recovery from primary infection, all dogs remained subclinical, did not shed virus, and had stable or declining CHV-1 SN titers.
Canine herpesvirus-1 primary ocular infection in adult dogs is associated with self-limiting conjunctivitis and ocular viral shedding, which occurs in the absence of clinically detectable keratitis or systemic disease. The relative magnitude of ocular lesions associated with experimental primary CHV-1 ocular infection in adult dogs is mild and the duration of viral shedding is short, in contrast to experimental alphaherpesvirus primary ocular infections in many other animal species.
This PDF is available to Subscribers Only