May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Anti-Inflammatory Effects of Besifloxacin, a Novel Fluoroquinolone, in Primary Human Corneal Epithelial Cells
Author Affiliations & Notes
  • M. E. Cavet
    Vision Care Preclinical Research, Bausch and Lomb, Rochester, New York
  • K. R. VanDerMeid
    Vision Care Preclinical Research, Bausch and Lomb, Rochester, New York
  • J. Zhang
    Vision Care Preclinical Research, Bausch and Lomb, Rochester, New York
  • K. W. Ward
    Vision Care Preclinical Research, Bausch and Lomb, Rochester, New York
  • Footnotes
    Commercial Relationships  M.E. Cavet, Bausch and Lomb, E; K.R. VanDerMeid, Bausch and Lomb, E; J. Zhang, Bausch and Lomb, E; K.W. Ward, Bausch and Lomb, E.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2500. doi:
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      M. E. Cavet, K. R. VanDerMeid, J. Zhang, K. W. Ward; Anti-Inflammatory Effects of Besifloxacin, a Novel Fluoroquinolone, in Primary Human Corneal Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2500.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The aim of this study was to determine the anti-inflammatory effects of besifloxacin, a novel fluoroquinolone under clinical evaluation for the treatment of ophthalmic infections, in human corneal epithelial cells.

Methods: : Cytokine expression in primary human corneal epithelial cells (HCEpiC) was stimulated by IL-1ß and Luminex technology was used to determine the effect of besifloxacin on IL-1ß-induced cytokine expression. Moxifloxacin, a marketed fluoroquinolone, was used as the control. NFkB activation was assessed by measuring IΚB degradation and NFΚB nuclear translocation by Western blotting.

Results: : IL-1ß induced measurable cytokine expression for 12 of the 14 cytokines detected. Besifloxacin significantly inhibited IL-1ß-stimulated cytokine production in a dose-dependent manner, with a comparable (G-CSF, GM-CSF, IL-6, IL-8, MCP-1, and MIP-1ß) or better (TGF-α and TNF-α) potency compared to moxifloxacin. A significant inhibitory effect of besifloxacin was observed at 1 or 10 µg/mL. Besifloxacin inhibited both IΚB degradation and NFΚB nuclear translocation.

Keywords: cornea: epithelium • antibiotics/antifungals/antiparasitics • cytokines/chemokines 
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