May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Elucidating the Inhibitory Mechanism of Müller Cells on T Lymphocyte Proliferation in vitro
Author Affiliations & Notes
  • R. C. Rigden
    Immunoregulation, National Eye Institute, NIH, Bethesda, Maryland
  • L. M. Cortes
    Immunoregulation, National Eye Institute, NIH, Bethesda, Maryland
  • D. Luger
    Immunoregulation, National Eye Institute, NIH, Bethesda, Maryland
  • M. Matapallil
    Immunoregulation, National Eye Institute, NIH, Bethesda, Maryland
  • P. B. Silver
    Immunoregulation, National Eye Institute, NIH, Bethesda, Maryland
  • R. R. Caspi
    Immunoregulation, National Eye Institute, NIH, Bethesda, Maryland
  • Footnotes
    Commercial Relationships  R.C. Rigden, None; L.M. Cortes, None; D. Luger, None; M. Matapallil, None; P.B. Silver, None; R.R. Caspi, None.
  • Footnotes
    Support  NIH Intramural funding
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2512. doi:https://doi.org/
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      R. C. Rigden, L. M. Cortes, D. Luger, M. Matapallil, P. B. Silver, R. R. Caspi; Elucidating the Inhibitory Mechanism of Müller Cells on T Lymphocyte Proliferation in vitro. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2512. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To examine the ability of murine Müller cells (MC) to inhibit activation and proliferation of T lymphocytes and identify the molecule(s) by which this effect occurs.

Methods: : MC from primary cultures established from wild type (WT) or genetically manipulated C57BL/6 mice were irradiated and co-cultured with sorted CD4+ T cells stimulated with anti CD3/CD28. Blocking Abs and supernatant transfers were used to study the surface molecule(s) involved in inhibition and its contact dependence. Regulatory phenotype and function of T cells retrieved from T/MC co-cultures were examined by expression of FoxP3 and CD25 and inhibition of naive stimulated T cells, respectively. Proliferation of T cells was measured by 3H-thymidine incorporation or by dilution of CFSE.

Results: : Murine MC strongly inhibited CD3/CD28 driven T cell proliferation in a contact dependent fashion. The use of TSP-1-/-, B7.1-/- and mice deficient in TGF-β signaling, as well as blocking antibodies to B7.1, TGF-β and TSP-1 revealed that interference with these molecules diminished, but did not completely abrogate the inhibitory effect. CD25 expression was inhibited and FoxP3 expression was not induced in CD4+ T cells by co- culture with MC. Regulatory function that could be attributed to an effect of MC was also not demonstrable.

Keywords: Muller cells • immunomodulation/immunoregulation • inhibitory receptors 
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