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Q. Meng, P. Yang, B. Li, H. Zhou, X. Huang; PD-1 Expression in the Development of Anterior Chamber-Associated Immune Deviation. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2515.
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To study the expression and possible implication of programmed death-1 (PD-1) in iris-ciliary body from mice with anterior chamber-associated immune deviation (ACAID).
ACAID was induced in BALB/c mice by the intracameral injection of 50µg ovalbumin (OVA) and evaluated by delayed-type hypersensitivity response. The expressions of PD-1 and its ligands in iris-ciliary bodies from ACAID mice were determined by fluorescent quantitative real-time polymerase chain reaction and immunohistochemistry.
Both mRNA and protein of PD-1 and PD-L1 were expressed in the iris-ciliary body. PD-1 mRNA and protein were up-regulated in ACAID mice, while PD-L1 mRNA and protein were up-regulated in positive control mice. Neither mRNA nor protein of PD-L2 was detected in the iris-ciliary body of the different groups.
An increased expression of PD-1 in iris-ciliary body of ACAID mice indicates its involvement in the induction of this immune deviation.
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