May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Reduced Inhibitory Function of Ocular Regulatory T Cells in Recurrent Autoimmune Uveitis
Author Affiliations & Notes
  • Y. Bian
    Ophthalmology and Visual Sciences, University of Louisville, Louisville, Kentucky
  • Y. Ke
    Ophthalmology and Visual Sciences, University of Louisville, Louisville, Kentucky
  • D. Sun
    Ophthalmology and Visual Sciences, University of Louisville, Louisville, Kentucky
  • G. Jiang
    Ophthalmology and Visual Sciences, University of Louisville, Louisville, Kentucky
  • H. J. Kaplan
    Ophthalmology and Visual Sciences, University of Louisville, Louisville, Kentucky
  • H. Shao
    Ophthalmology and Visual Sciences, University of Louisville, Louisville, Kentucky
  • Footnotes
    Commercial Relationships  Y. Bian, None; Y. Ke, None; D. Sun, None; G. Jiang, None; H.J. Kaplan, None; H. Shao, None.
  • Footnotes
    Support  NIH grants EY12974, EY14599, EY014366, EY017373, R24 EY015636 and RBP career development award (HS).
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2516. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Y. Bian, Y. Ke, D. Sun, G. Jiang, H. J. Kaplan, H. Shao; Reduced Inhibitory Function of Ocular Regulatory T Cells in Recurrent Autoimmune Uveitis. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2516.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To determine whether CD4+CD25+ T regulatory cells (Tregs) from the eyes of rats with recurrent EAU (r-EAU) were less efficient in suppressing intraocular inflammation than those from rats with monophasic disease (m-EAU).

Methods: : m-EAU and r-EAU were induced in Lewis rats either by immunization with R16 or by adoptive transfer of R16-specific T cells, respectively. Ocular CD4+CD25+ Tregs were separated from CD4+ CD25- T effector cells and the inhibitory functions of Tregs determined. Aqueous humor (AqH) from m-EAU and r-EAU were collected and studied for their ability to enhance ocular Treg function.

Results: : We found that the number of ocular CD4+CD25+ (Tregs) increased in the eye during resolution of the first acute attack of intraocular inflammation in both m-EAU and r-EAU. However, the suppressor function of these cells was much weaker in r-EAU. The suppressor function of ocular Tregs in r-EAU was enhanced by incubation with AqH from animals recovering from m-EAU. Moreover, the weaker suppressor function of ocular Tregs in r-EAU correlated with low or undetectable levels of IL-10 in the AqH, and was reversed by the addition of IL-10 to the AqH. Finally, the transfer of ocular Tregs from animals with m-EAU converted r-EAU into a monophasic disease.

Conclusions: : Our study demonstrated that although a number of mechanisms may contribute to the recurrence of intraocular inflammation, dysregulation and malfunction of Tregs in the eye is an important factor in disease recurrence.

Keywords: autoimmune disease • immunomodulation/immunoregulation 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×