Purchase this article with an account.
Y. Bian, Y. Ke, D. Sun, G. Jiang, H. J. Kaplan, H. Shao; Reduced Inhibitory Function of Ocular Regulatory T Cells in Recurrent Autoimmune Uveitis. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2516.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To determine whether CD4+CD25+ T regulatory cells (Tregs) from the eyes of rats with recurrent EAU (r-EAU) were less efficient in suppressing intraocular inflammation than those from rats with monophasic disease (m-EAU).
m-EAU and r-EAU were induced in Lewis rats either by immunization with R16 or by adoptive transfer of R16-specific T cells, respectively. Ocular CD4+CD25+ Tregs were separated from CD4+ CD25- T effector cells and the inhibitory functions of Tregs determined. Aqueous humor (AqH) from m-EAU and r-EAU were collected and studied for their ability to enhance ocular Treg function.
We found that the number of ocular CD4+CD25+ (Tregs) increased in the eye during resolution of the first acute attack of intraocular inflammation in both m-EAU and r-EAU. However, the suppressor function of these cells was much weaker in r-EAU. The suppressor function of ocular Tregs in r-EAU was enhanced by incubation with AqH from animals recovering from m-EAU. Moreover, the weaker suppressor function of ocular Tregs in r-EAU correlated with low or undetectable levels of IL-10 in the AqH, and was reversed by the addition of IL-10 to the AqH. Finally, the transfer of ocular Tregs from animals with m-EAU converted r-EAU into a monophasic disease.
Our study demonstrated that although a number of mechanisms may contribute to the recurrence of intraocular inflammation, dysregulation and malfunction of Tregs in the eye is an important factor in disease recurrence.
This PDF is available to Subscribers Only