May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Surrogate Acaid Antigen Presenting Cells Induce Ly49c/i Dependent Il-10 Production in INkt Cells
Author Affiliations & Notes
  • R. Mathew
    Schepens Eye Research Institute, Boston, Massachusetts
    Immunology,
  • C. Watte
    Schepens Eye Research Institute, Boston, Massachusetts
    Immunology,
  • C. Kan
    Schepens Eye Research Institute, Boston, Massachusetts
    Harvard Immunology Summer Program,
  • J. Stein-Streilein
    Schepens Eye Research Institute, Boston, Massachusetts
    Immunology,
  • Footnotes
    Commercial Relationships  R. Mathew, None; C. Watte, None; C. Kan, None; J. Stein-Streilein, None.
  • Footnotes
    Support  NIH grant : EY0111983
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2520. doi:https://doi.org/
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      R. Mathew, C. Watte, C. Kan, J. Stein-Streilein; Surrogate Acaid Antigen Presenting Cells Induce Ly49c/i Dependent Il-10 Production in INkt Cells. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2520. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : NKT cells express various NK cell-associated molecules including proteins in the Ly49 receptor family. Ly49 C/I is an inhibitory receptor that when ligated inhibits cytolytic activity and cytokine production by NK and NKT cells Recently, in vivo studies in our laboratory established that Ly49 C/I invariant (i)NKT cells were required for induction of peripheral tolerance via the eye (Anterior chamber associated immune deviation, ACAID).The purpose of this study was to analyze the role of Ly49 C/I on iNKT cells in the production of immunosuppressive cytokines (IL-10) post exposure to tolerogenic APC( surrogate ACAID APC).

Methods: : Antigen presenting cells (APC) were derived from either peritoneal exudate cells (PEC) or bone marrow cell cultures. Incubation of the APC with OVA or TGF β and OVA generated immunogenic or tolerogenic APC, respectively The resulting APC were then incubated with iNKT cells ( 24.8A hybridoma) for varying time periods with or without anti-LY49 (5E6). FACS analysis, ELISA and semi-quantitative RT-PCR were used to measure protein expression and mRNA expression of cytokines (IL-10 and IFNγ) relative to the house keeping genes.

Results: : iNKT cells (24.8A hybridoma) showed an increase in IL-10 mRNA expression and decrease in IFNγ after one hour incubation with tolerogenic APC. iNKT cells responded to immunogenic APC with increased IFNγ mRNA and little or no IL-10 mRNA levels.

Conclusions: : These results support the postulate that ligation of Ly49 C/I on iNKT cells or their hybridoma counterparts not only inhibits inflammatory cytokine ( IFNγ) production, but at the same time contributes to the production of immunosuppressive cytokines, such as IL-10. Thus the role of Ly49 C/I is enlarged and includes this novel role in the induction of peripheral tolerance. The research was supported in part by NIH grant : EY0111983

Keywords: cytokines/chemokines • immune tolerance/privilege • immunomodulation/immunoregulation 
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