May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Exposure of Retinal Astrocytes to IL-17 Elicited Massive Expression of Cytokines and Chemokines That Recruit Non Lymphoid Inflammatory Cells
Author Affiliations & Notes
  • Y. Ke
    Department of Ophthalmology, University of Louisville, Louisville, Kentucky
  • D. Sun
    Department of Ophthalmology, University of Louisville, Louisville, Kentucky
  • G. Jiang
    Department of Ophthalmology, University of Louisville, Louisville, Kentucky
  • Y. Bian
    Department of Ophthalmology, University of Louisville, Louisville, Kentucky
  • H. J. Kaplan
    Department of Ophthalmology, University of Louisville, Louisville, Kentucky
  • H. Shao
    Department of Ophthalmology, University of Louisville, Louisville, Kentucky
  • Footnotes
    Commercial Relationships  Y. Ke, None; D. Sun, None; G. Jiang, None; Y. Bian, None; H.J. Kaplan, None; H. Shao, None.
  • Footnotes
    Support  NIH grants EY12974, EY14599, EY014366, EY017373, R24 EY015636 and RBP career development award (HS).
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2528. doi:https://doi.org/
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    • Get Citation

      Y. Ke, D. Sun, G. Jiang, Y. Bian, H. J. Kaplan, H. Shao; Exposure of Retinal Astrocytes to IL-17 Elicited Massive Expression of Cytokines and Chemokines That Recruit Non Lymphoid Inflammatory Cells. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2528. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : We have previously shown that autoreactive IL-17 producing T cells could induce experimental autoimmune uveitis (EAU). However, the mechanisms of IL-17 in the pathogenic events of uveitis are largely unknown. To determine the pathogenic events induced by IL-17, we have examined the effect of IL-17 on the parenchymal cells of the retinal astrocytes and RPE.

Methods: : Retinal astrocytes and RPE were isolated as we previously reported. The cells were exposed to graded doses of IL-17. The cytokine/chemokines in the supernatants of astrocytes and RPE were examined by ELISA. The chemotaxis of the supernatants to the migration of T cells, granulocytes, or monocytes/macrophages was determined. Signal transduction pathways involved in the interaction of IL-17 and IL-17R on these cells were examined.

Results: : Both RACs and RPE express IL-17 receptor; however, only astrocytes vigorously responded to IL-17 by producing increased amounts of cytokines and chemokines, which promoted the migration of granulocytes, monocytes and macrophages, but not T cells. The interaction of IL-17/IL-17R on RACs is mainly dependent on PI-3K/AKT signaling pathway.

Conclusions: : IL-17 produced by the IL-17+ IRBP-specific T cells can activate local parenchymal cells such as astrocytes and activated astrocytes release abundant amounts of cytokines and chemokines, which recruit non-lymphoid inflammatory cells, in turn, amplify inflammatory cascades in the eye.

Keywords: astrocyte • autoimmune disease 
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