May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Vasodilatory Effect of Antiglaucomatous Prostaglandin Analogues on Isolated Rabbit and Mouse Ciliary Arteries
Author Affiliations & Notes
  • H. Watabe
    Ophthalmology, Akita University, Akita, Japan
  • N. Sato
    Ophthalmology, Akita University, Akita, Japan
  • S. Takaseki
    Ophthalmology, Akita University, Akita, Japan
  • T. Yoshitomi
    Ophthalmology, Akita University, Akita, Japan
  • H. Ishikawa
    Ophthalmology, Kitasato University, Kanagawa, Japan
  • Footnotes
    Commercial Relationships  H. Watabe, Santen Phamaceutical Co., Ltd, F; N. Sato, Santen Phamaceutical Co., Ltd., F; S. Takaseki, Santen Phamaceutical Co., Ltd., F; T. Yoshitomi, Santen Phamaceutical Co., Ltd., F; H. Ishikawa, None.
  • Footnotes
    Support  Japan Grant-in-Aid for Scientific Research #12671719
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2615. doi:https://doi.org/
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    • Get Citation

      H. Watabe, N. Sato, S. Takaseki, T. Yoshitomi, H. Ishikawa; Vasodilatory Effect of Antiglaucomatous Prostaglandin Analogues on Isolated Rabbit and Mouse Ciliary Arteries. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2615. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Topical application of antiglaucomatous prostaglandin (PG) analogues, Latanoprost, Unoprostone and Tafluprost, were reported to increase ocular blood flow. To investigate the vasodilatory mechanism of these drugs, we have investigated the effect of these drugs on isolated rabbit and mouse ciliary arteries in vitro.

Methods: : Under the dissecting microscope, ciliary arteries were prepared from albino rabbit eyes and mounted in a myograph system. The effects of PG analogues were investigated using isometric tension recoding methods. We further investigated the effects of these drugs on changes in intracellular Ca2+ concentrations ([Ca2+]i) in mouse ciliary arteries by using the Ca2+-sensitive dye fura-2 as an indicator.

Results: : These three PG analogues induced dose-dependent relaxations in rabbit ciliary artery that were pre-contracted with high-K solution. Application of NG-nitro-L-arginine methylester (300µM) or indomethacin (10µM) did not affect this relaxation. These three PG analogues significantly decreased the [Ca2+]i in mouse ciliary artery elevated by high-K.

Conclusions: : Antiglaucomatous PG analogues relaxed and decreased [Ca2+]i in rabbit or mouse ciliary arteries. The mechanism of vascular smooth muscle relaxation by PG analogues does not depend on NO or intrinsic PG production.

Keywords: blood supply • calcium • nitric oxide 
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