May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Immunohistochemistry and in situ Zymography to Assess Matrix Metalloproteinases Expression and Activation by Latanoprost in Rat Uveoscleral Pathway
Author Affiliations & Notes
  • P.-P. Elena
    Iris Pharma, La Gaude, France
  • A. Freton
    Iris Pharma, La Gaude, France
  • A. Spadafora
    Iris Pharma, La Gaude, France
  • S. Grillo-Antonelli
    Iris Pharma, La Gaude, France
  • N. Cimbolini
    Iris Pharma, La Gaude, France
  • L. Feraille
    Iris Pharma, La Gaude, France
  • M. Lazdunski
    Institut de Pharmacologie Moléculaire et Cellulaire, CNRS, Sophia Antipolis, France
  • Footnotes
    Commercial Relationships  P. Elena, None; A. Freton, None; A. Spadafora, None; S. Grillo-Antonelli, None; N. Cimbolini, None; L. Feraille, None; M. Lazdunski, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2616. doi:https://doi.org/
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      P.-P. Elena, A. Freton, A. Spadafora, S. Grillo-Antonelli, N. Cimbolini, L. Feraille, M. Lazdunski; Immunohistochemistry and in situ Zymography to Assess Matrix Metalloproteinases Expression and Activation by Latanoprost in Rat Uveoscleral Pathway. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2616. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Rodents glaucoma models become of widespread use. In such species though, uveoscleral pathway function remains partially unknown. The aim of the study was to evaluate the matrix metalloproteinases (MMP) 2 and 9 topography of expression and activation within rat ciliary muscle following latanoprost treatment.

Methods: : A single dose of 500 ng of latanoprost (Xalatan®) or vehicle was applied topically in right eye of albino rats (wistar). Ocular biospecimen were collected at different time-points after instillation. MMP-2, MMP-9 expression and gelatinolytic activity were assessed on frozen sections by immunohistochemistry and in situ zymography, respectively.

Results: : Latanoprost treatment enhanced MMP-2 and MMP-9 expression in the sclera and in area adjacent to the ciliary muscle 24 hours after instillation. An increase of gelatinolytic activity was observed in colocalisation with MMP-9 expression.

Conclusions: : Latanoprost induced MMP-2 and MMP-9 expression and activation within rat during uveoscleral pathway regulation. In situ assessment of MMPs expression and activation in response to latanoprost contributed to a better understanding of rodents uveoscleral pathway regulation, which would be relevant and well correlated with human pathology.

Keywords: immunohistochemistry • glycoconjugates/glycoproteins • gene/expression 
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