May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Role of Prostanoids in the Inhibitory Effect of 8-Isoprostaglandin E2 on Bovine Retinal Glutamate and Glycine Levels, ex vivo
Author Affiliations & Notes
  • C. A. Opere
    Pharmacy Sciences, Creighton University, Omaha, Nebraska
  • M. Zhao
    Pharmacy Sciences, Creighton University, Omaha, Nebraska
  • C. J. Destache
    Pharmacy Sciences, Creighton University, Omaha, Nebraska
  • S. E. Ohia
    College of Pharmacy, University of Houston, Houston, Texas
  • Footnotes
    Commercial Relationships  C.A. Opere, None; M. Zhao, None; C.J. Destache, None; S.E. Ohia, None.
  • Footnotes
    Support  NIH EY 013967
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2623. doi:https://doi.org/
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      C. A. Opere, M. Zhao, C. J. Destache, S. E. Ohia; Role of Prostanoids in the Inhibitory Effect of 8-Isoprostaglandin E2 on Bovine Retinal Glutamate and Glycine Levels, ex vivo. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2623. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The aim of the present study was to investigate the role of prostanoids in the inhibitory effect of 8-isoPGE2 on endogenous amino acid neurotransmitter levels in bovine retina, ex vivo.

Methods: : Freshly isolated bovine eyeballs were equilibrated in oxygenated Krebs buffer solution before being injected intravitreally with either 8-IsoPGE2 or vehicle for control. After 30 minutes of incubation in Krebs buffer solution, retina was isolated and prepared for measurement of glutamate, glutamine and glycine by HPLC-EC. When used, cyclooxygenase (COX) and thromboxane (Tx) synthase inhibitors were intravitreally injected into the eyeballs 15 minutes prior to application of 8-isoPGE2.

Results: : Intravitreally administered 8-isoPGE2 (0.1 nM - 100 µM) reduced the levels of the excitatory neurotransmitter, glutamate and its metabolite, glutamine and the inhibitory neurotransmitter, glycine in a concentration-dependent manner in bovine retina, ex vivo, yielding IC35 values of 1.5 µM, 5.0 µM and 100 µM, respectively. The non-selective COX inhibitor, flurbiprofen and COX-2 selective inhibitor, NS-398 had no significant effect (p>0.05) on the endogenous levels of glutamate, glutamine, and glycine in bovine retina. Furthermore, flurbiprofen (10 µM) and NS-398 (10 µM) had no effect (P>0.05) on 8-isoPGE2 (0.1 nM - 100 µM)-induced attenuation of glutamate, glutamine and glycine levels. Similarly, the Tx-synthase inhibitor, furegrelate (10 µM) had no effect (P>0.05) on endogenous amino acid levels and did not reverse the isoprostane-induced inhibition of glutamate, glutamine and glycine.

Keywords: neurotransmitters/neurotransmitter systems • retina • receptors: pharmacology/physiology 
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