May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Comprehensive Gene Expression Profiles in Murine Oxygen-induced Retinopathy
Author Affiliations & Notes
  • T. Sato
    Osaka Univ Grad Sch of Med, Suita, Japan
    Applied Visual Science,
  • S. Kusaka
    Osaka Univ Grad Sch of Med, Suita, Japan
    Applied Visual Science,
  • N. Hashida
    Osaka Univ Grad Sch of Med, Suita, Japan
    Ophthalmology,
  • Y. Saishin
    Osaka Univ Grad Sch of Med, Suita, Japan
    Ophthalmology,
  • T. Fujikado
    Osaka Univ Grad Sch of Med, Suita, Japan
    Applied Visual Science,
  • Y. Tano
    Osaka Univ Grad Sch of Med, Suita, Japan
    Ophthalmology,
  • Footnotes
    Commercial Relationships  T. Sato, None; S. Kusaka, None; N. Hashida, None; Y. Saishin, None; T. Fujikado, None; Y. Tano, None.
  • Footnotes
    Support  Grant from the Japanese Ministry of Education, Culture, Sports, Science, and Technology (No, 17591832)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2626. doi:https://doi.org/
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      T. Sato, S. Kusaka, N. Hashida, Y. Saishin, T. Fujikado, Y. Tano; Comprehensive Gene Expression Profiles in Murine Oxygen-induced Retinopathy. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2626. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate a comprehensive expression profile of genes in murine oxygen-induced retinopathy (OIR), and to correlate the expression pattern of the genes with the clinical time course of OIR.

Methods: : OIR was induced in C57BL/6 mice by placing postnatal day 7 (P7) pups in 75% oxygen for 5 days. The time course of OIR was evaluated daily on retinal flat-mounts from P12 to P21. Microarray analysis was performed on OIR and control retinas on P17 to select the candidates for TaqMan® low-density array (TLDA). Real-time PCR with TLDA was performed by comparative CT method at P12 to P21, and the successive expression patterns of the genes in OIR were analyzed by hierarchical clustering.

Results: : Microarray analysis showed that 25 probes associated with angiogenesis were up-regulated and 3 probes were down-regulated in murine OIR. TLDA cluster analysis revealed a homology of gene expression patterns between P12 and P13 and between P16 and P17. Many genes associated with inflammation were up-regulated from the beginning when the degrees of both central avascular area and central vasoconstriction were maximal, and the up-regulation of the genes continued to the late stage. Several genes associated with angiogenesis, such as angiopoietin-2 and vascular endothelial growth factor-A, were remarkably up-regulated around P16 and P17 when extraretinal neovascularization became most noticeable.

Conclusions: : An increase in the expression of genes associated with inflammation preceded the angiogenesis and the up-regulation of genes associated with angiogenesis. The gene expression patterns were well-correlated with the clinical presentations. These findings will be valuable for the understanding of the pathological conditions involved in angiogenesis.

Keywords: retinopathy of prematurity • retinal neovascularization • gene/expression 
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