May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Differential Expression of Pro-Angiogenic Factors in Physiological and Pathological Angiogenesis of the Retina
Author Affiliations & Notes
  • G. Kim
    Seoul National University Hospital, Seoul, Republic of Korea
    Dep of Pediatrics,
  • J. Kim
    Seoul National University Hospital, Seoul, Republic of Korea
    Ophthalmology,
  • J. Kim
    Seoul National University Hospital, Seoul, Republic of Korea
    Ophthalmology,
  • Y. Yu
    Seoul National University Hospital, Seoul, Republic of Korea
    Ophthalmology,
  • K.-W. Kim
    Pharmacy, Seoul National University, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  G. Kim, None; J. Kim, None; J. Kim, None; Y. Yu, None; K. Kim, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2635. doi:
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    • Get Citation

      G. Kim, J. Kim, J. Kim, Y. Yu, K.-W. Kim; Differential Expression of Pro-Angiogenic Factors in Physiological and Pathological Angiogenesis of the Retina. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2635.

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Abstract

Purpose: : To investigate the differential expression of pro-angiogenic factors in physiological and pathological angiogenesis of the retina

Methods: : On post-natal (P) P7, P14, and P18 of control mice, and P14, and P18 of oxygen-induced retinopathy (OIR) mice, the eyes were enucleated. The vascular formation in the physiological and pathological angiogenesis of the retina was evaluated through flourescein angiography using FITC-dextran. Immunohistochemistry for vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF)-2, platelet-derived growth factor (PDGF)-B, insulin-like growth factor (IGF)-1/-2, and hepatocyte growth factor (HGF) was performed on cross-sections of the control and OIR retina. In addition, western blot analysis for each factor was done using whole retina.

Results: : In the control retina, VEGF and PDGF-B were primarily expressed on endothelial cells (ECs) of retinal vessels. Although the expressions of FGF-2, IGF-1/-2 and HGF were also detected on ECs, the immunereactivities were very weak. Interestingly, the expression of IGF-2 was confined to the endothelial cells of retinal vessels as our previous report. In OIR, the immunereactivities of VEGF, PDGF-B, and HGF were increased, however other factors showed no definite change in the immunoreactivities.

Conclusions: : Our data suggests that the physiological and pathological angiogenesis of the retina might be dependent on the differential expression of pro-angiogenic factors in the retina.

Keywords: retinal neovascularization • growth factors/growth factor receptors • neovascularization 
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