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G. Kim, J. Kim, J. Kim, Y. Yu, K.-W. Kim; Differential Expression of Pro-Angiogenic Factors in Physiological and Pathological Angiogenesis of the Retina. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2635.
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To investigate the differential expression of pro-angiogenic factors in physiological and pathological angiogenesis of the retina
On post-natal (P) P7, P14, and P18 of control mice, and P14, and P18 of oxygen-induced retinopathy (OIR) mice, the eyes were enucleated. The vascular formation in the physiological and pathological angiogenesis of the retina was evaluated through flourescein angiography using FITC-dextran. Immunohistochemistry for vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF)-2, platelet-derived growth factor (PDGF)-B, insulin-like growth factor (IGF)-1/-2, and hepatocyte growth factor (HGF) was performed on cross-sections of the control and OIR retina. In addition, western blot analysis for each factor was done using whole retina.
In the control retina, VEGF and PDGF-B were primarily expressed on endothelial cells (ECs) of retinal vessels. Although the expressions of FGF-2, IGF-1/-2 and HGF were also detected on ECs, the immunereactivities were very weak. Interestingly, the expression of IGF-2 was confined to the endothelial cells of retinal vessels as our previous report. In OIR, the immunereactivities of VEGF, PDGF-B, and HGF were increased, however other factors showed no definite change in the immunoreactivities.
Our data suggests that the physiological and pathological angiogenesis of the retina might be dependent on the differential expression of pro-angiogenic factors in the retina.
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