May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Retinal Vein Occlusion in Pigs as a Model of Acute Retinal Edema
Author Affiliations & Notes
  • J. Combal
    R & D, FOVEA Pharmaceuticals, Paris, France
  • D. Pruneau
    R & D, FOVEA Pharmaceuticals, Paris, France
  • B. El-Mathari
    R & D, FOVEA Pharmaceuticals, Paris, France
  • M. Paques
    INSERM U592, Paris, France
  • A.-J. Sahel
    INSERM U592, Paris, France
  • Footnotes
    Commercial Relationships  J. Combal, None; D. Pruneau, None; B. El-Mathari, None; M. Paques, None; A. Sahel, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2685. doi:https://doi.org/
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      J. Combal, D. Pruneau, B. El-Mathari, M. Paques, A.-J. Sahel; Retinal Vein Occlusion in Pigs as a Model of Acute Retinal Edema. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2685. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Retinal edema associated with vein occlusion (RVO) is a leading cause of visual loss in humans, yet there is a paucity of clinically relevant animal models of such edemaWe developed a pig model of post-RVO acute retinal edema to evaluate potential pharmacological interventions.

Methods: : RVO was induced by transvitreal cauterization of a branch retinal vein in 34 eyes of adult farm pigs. At 24h, blood-retinal barrier rupture was quantified by optical coherence tomography and post-mortem measurement of Evans Blue (EB) dye content.

Results: : At 24h, complete occlusion of the vein was observed in 85% of eyes. In occluded eyes, fundus examination showed extensive retinal haemorrhages, blebs and folds upstream of the occlusion site. Fluorescein angiography revealed widespread capillary closure and leakage. Retinal thickness was increased (mean (±SD) 404 ± 19 µm vs. 190 ± 4 µm ; P<0.001) and EB dye content was higher in RVO eyes (9.4 ± 0.7 µl plasma x g retina wet weight-1 hr-1) as compared to sham-operated eyes (3.2 ± 0.6) and control animals (2.1 ± 0.6; P<0.001). Similar differences were found in the vitreous (2.0 ± 0.3 vs. 0.19 ± 0.04; P<0.001). In the 4 eyes followed up 3 weeks, retinal atrophy was observed.

Keywords: edema • vascular occlusion/vascular occlusive disease • imaging/image analysis: non-clinical 
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