May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Therapeutic Effects of a Novel Compound, CLT003, in Diabetic Retinopathy and Age-Related Macular Degeneration
Author Affiliations & Notes
  • Y. Hu
    OUHSC, Oklahoma City, Oklahoma
  • Y. Chen
    OUHSC, Oklahoma City, Oklahoma
  • R. Mott
    OUHSC, Oklahoma City, Oklahoma
  • K. Lu
    OUHSC, Oklahoma City, Oklahoma
  • P.-K. Li
    the Ohio State, Columbus, Ohio
  • J.-X. Ma
    OUHSC, Oklahoma City, Oklahoma
  • D. Chen
    Charlesson LLC, Oklahoma City, Oklahoma
  • Footnotes
    Commercial Relationships  Y. Hu, None; Y. Chen, None; R. Mott, None; K. Lu, None; P. Li, None; J. Ma, None; D. Chen, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2747. doi:https://doi.org/
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      Y. Hu, Y. Chen, R. Mott, K. Lu, P.-K. Li, J.-X. Ma, D. Chen; Therapeutic Effects of a Novel Compound, CLT003, in Diabetic Retinopathy and Age-Related Macular Degeneration. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2747. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Abnormal retinal vascular leakage is on of the major pathological changes in diabetic retinopathy (DR) and age-related macular degeneration (AMD). With a goal of developing drugs to treat retinal vascular leakage, we have synthesized a series of novel thalidomide analogs and evaluated their inhibitory effects on NV and retinal vascular leakage. The purpose of this study is to evaluate the efficacy of CLT-003 which has exhibited a potent anti-angiogenic activity.

Methods: : Human Umbilical Vein Endothelial Cells (HUVEC) were grown in the EBM-MV2 medium. Bovine Retinal Endothelial Cells (BREC) and pericytes were isolated from fresh cow eyes. MTT was used to quantify the cell viability. This fluorescence-based endothelial cell invasion assay used on BD MatrigelTM and BD FalconTM HTS FluoroBlokTM 24-Multiwell Insert System. Newborn Brown Norway (BN) rats at postnatal day 7 were exposed to hyperoxia (75% O2) for 5 days and then returned to normoxia to induce oxygen-induced retinopathy. Adult BN rats were given a single intraperitoneal injection of streptozotocin (STZ) to induce diabetes. Vascular permeability was quantified by measuring leakage of FITC-albumin or Evans blue dye-albumin complex from the blood vessels into the retina. Full-field electroretinogram (ERG) was recorded using the Espion E2 ERG system.

Results: : CLT003 and thalidomide reduced retinal vascular leakage in a dose-dependent manner from 0.5 to 1.0 µg/eye in both the OIR and STZ-diabetes models. CLT003 has significantly more potent effects on retinal vascular leakage, when compared with thalidomide and other know thalidomide analogs after a single intravitreal injection. Additionally, CLT003 blocks retinal vascular leakage in an AMD mouse model. Further, CLT003 down-regulated the expression of VEGF and ICAM-1 in the retina, suggesting that the anti-vascular leakage activity of CLT003 is, at least in part, through blocking VEGF production and down-regulating inflammatory factors. ERG and histological analysis at various intervals after the drug administration showed that a single intravitreal injection of CLT003 did not cause detectable functional and morphological abnormalities in ocular tissues.

Conclusions: : CLT003 is a novel analog of thalidomide with potent vascular activities. It has therapeutic potential in retinal vascular leakage in DR and AMD.

Keywords: diabetic retinopathy • drug toxicity/drug effects • ischemia 
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