May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Loss of Tenascin-c Perturbs the Epithelial-Mesenchymal Transition in an Injured Mouse Lens Epithelium
Author Affiliations & Notes
  • S. Tanaka
    Dept Ophthalmology, Wakayama Medical University, Wakayama, Japan
  • K.-I. Miyazaki
    Dept Ophthalmology, Wakayama Medical University, Wakayama, Japan
  • S. Saika
    Dept Ophthalmology, Wakayama Medical University, Wakayama, Japan
  • Footnotes
    Commercial Relationships  S. Tanaka, None; K. Miyazaki, None; S. Saika, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2774. doi:https://doi.org/
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      S. Tanaka, K.-I. Miyazaki, S. Saika; Loss of Tenascin-c Perturbs the Epithelial-Mesenchymal Transition in an Injured Mouse Lens Epithelium. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2774. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigated the role of tenascin-C in epithelial-mesenchymal transition (EMT) of the lens epithelium during wound healing in mice.

Methods: : The crystalline lens in one eye was injured by needle puncture in tenascin-C-null (KO, n = 40) and wild-type (WT, n = 40) mice under both general and topical anesthesia. The animals were killed at day 1, 2, 5 and 10 post-injury. Immunohistochemistry was employed to detect tenescin-C, α-smooth muscle action (alpha-SMA), a marker of EMT, collagen type I, transforming growth factor β1 (TGFβ1), TGFβ2.

Results: : WT lens epithelial cells up-regulate tenascin C and the underwent EMT post-puncture injury as evaluated by histology and immunohistochemistry for alpha-SMA or tenascin C. At up to day 2 both WT and KO lens epithelium exhibited an epithelilal shape and lacked alpha-SMA expression. At day 5 WT lens epithelial cells exhibited elongated fibroblastic morphology with marked expressed alpha-SMA, while KO cells were still of epithelium in histology and lacked alpha-SMA expression. At day 10 both WT and KO cells showed an elongated fibroblastic appearance with alpha-SMA expression, indicating an establishment of EMT.

Conclusions: : Injury-induced EMT of mouse lens epithelium and expression of collagen I and TGFβ were attenuated by lacking tenascin C

Keywords: EMT (epithelial mesenchymal transition) • pathobiology • wound healing 
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