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K. S. Johar, A. R. Vasavada; Cells of Human Anterior Capsular Plaques. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2775.
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© ARVO (1962-2015); The Authors (2016-present)
To study the differentiation status of cells in the human capsular plaques obtained from eyes undergoing cataract surgery.
Anterior capsular plaques were collected from adult and pediatric patients undergoing cataract surgery. 5 µm thick sections of these plaques were subjected to the localization of lens epithelial cells differentiation markers pax6, α-smooth muscle actin (α-SMA) and β-crystallin along with collagen type I, collagen type IV and αA-crystallin.
The lens epithelium obtained from patients revealed non-plaque and plaque region. The non-plaque region was largely consisted of pax6 positive cells. Within the plaque region, most of the cells attached to the basement membrane were positive to pax6. Among the cells suspended in the extracellular matrix of collagen type I, several cells were positive to α-SMA while some other cells were positive to β-crystallin. The cells of plaque were surrounded by a network of collagen type IV and αA-crystallin. Depending on the area, plaque region was classified into small, medium and large. Going from smaller to larger plaques, the number of cells positive to α-SMA and β-crystallin increased while the cells attached to the capsule remained positive to pax6.
Human capsular plaques consisted of heterogeneous population of undifferentiated and differentiated population of cells. Among the differentiated population an attempt was seen for both epithelial mesenchymal transition and fiber cell differentiation. The study is useful to determine the role of lens epithelial differentiation into the genesis of the human capsular plaques.
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