DBA/2J Mice as a Model of Cataractogenesis

Abstract

Purpose: : DBA/2J mice develop spontaneously changes in anterior chamber like pigment dispersion syndrome, iris atrophy, posterior synechiae leading to IOP increase. Additionally, there are observations suggesting cataract formation. The aim of this study was to describe features of cataract formation in DBA/2J mice. Moreover, presence and pattern of the L- kynurenine aminotransferases (KAT I and II) immunoreactivity in the cataractous lens of DBA/2J mice was investigated.

Methods: : Immunohistochemistry was conducted using polyclonal antibodies against KAT I and KAT II on 18 cataractous lenses of 8-, 11- and 24-month old DBA/2J mice (6 in each group). All the sections of the lens were stained with Periodic Acid - Schiff (PAS), and normal anatomy of the anterior segment of the eye was confirmed. Moreover, all the animals subjected to the study were examined using slit lamp and anterior chamber photography.

Results: : As observed clinically, the animals developed cataract during aging. PAS staining revealed presence of lens opacification. Immunohistochemical analysis revealed presence of KAT I and KAT II in the extracellular structures of cataract showing specific pattern of the stain. In fibrous metaplasia, immunoreactivity was concentrated between epithelial cells underlying the anterior capsule. In cortical cataract the immunoreactivity was observed on cortical lens fibres. In nuclear cataract, KAT II revealed stronger and diffused staining than KAT I. Additionally both KATs showed more pronounced staining at the edge of small clefts.

Conclusions: : Manifestation of L-kynurenine aminotransferases in extracellular matrix during cataract formation in DBA/2J mice suggests that products of L-kynurenine pathway might be involved in mechanisms of cataractogenesis in this animal model.

Keywords: cataract • immunohistochemistry • extracellular matrix