May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Validation of Human and Rabbit Cultured Corneal Epithelial Cell Sheets by Full-Field Optical Coherence Tomography
Author Affiliations & Notes
  • A. Kubota
    Ophthalmology and Visual Science, Tohoku Univ Graduate Sch of Med, Sendai, Miyagi, Japan
  • M. Akiba
    Topcon Advanced Biomedical Imaging Lab, Topcon Medical Systems, Paramus, New Jersey
  • Y. Takayanagi
    Ophthalmology and Visual Science, Tohoku Univ Graduate Sch of Med, Sendai, Miyagi, Japan
  • T. Soma
    Ophthalmology, Osaka Univ Med School, Suita, Osaka, Japan
  • N. Maeda
    Ophthalmology, Osaka Univ Med School, Suita, Osaka, Japan
  • C. Kimpui
    Topcon Advanced Biomedical Imaging Lab, Topcon Medical Systems, Paramus, New Jersey
  • K. Nishida
    Ophthalmology and Visual Science, Tohoku Univ Graduate Sch of Med, Sendai, Miyagi, Japan
  • Footnotes
    Commercial Relationships  A. Kubota, None; M. Akiba, Topcon Medical Systems, E; Y. Takayanagi, None; T. Soma, None; N. Maeda, None; C. Kimpui, Topcon Medical Systems, E; K. Nishida, None.
  • Footnotes
    Support  The Grants-in-Aid for Scientific Research (15390539, 16200036 and 16300161) in Japan
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2811. doi:https://doi.org/
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    • Get Citation

      A. Kubota, M. Akiba, Y. Takayanagi, T. Soma, N. Maeda, C. Kimpui, K. Nishida; Validation of Human and Rabbit Cultured Corneal Epithelial Cell Sheets by Full-Field Optical Coherence Tomography. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2811. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To observe the morphology and thickness of cultured human and rabbit corneal epithelial cell sheets alive and non-contact by use of a prototype full-field optical coherence tomography (FF-OCT) setup that offers ultrahigh (<1µm), three dimensional optical sectioning capability.

Methods: : Human and rabbit corneal epithelial cell sheets were prepared using the method that we previously reported. For the preparation of lethally treated National Institutes of Health (NIH)/3T3 feeder layers, subconfluent NIH/3T3 fibroblasts were incubated with 16 _g/mL of mitomycin C (MMC) for 2 hours at 37°C, and then trypsinized and seeded onto the temperature-responsive culture dishes at a density of 2x104 cells/cm2. Both the cultured sheets and the nucleated rabbit corneal epithelium were imaged by using the FF-OCT setup.

Results: : Basal cells, wing cells, and superficial cells in both enucleated rabbit cornea and cultured corneal cell sheets were observed at a cell level with the FF-OCT. The growth process of epithelial cell sheet could be monitored using the FF-OCT setup, and the thickness of the cultured epithelial sheet was measured quantitatively.

Conclusions: : FF-OCT is feasible for a cellular level observation of cultured epithelial cell sheet alive and non-contact. It may provide a useful mean for the validation of cultured epithelial cell sheets in regenerative medicine.

Keywords: cornea: clinical science • cornea: epithelium • imaging/image analysis: clinical 
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