May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
The Effect of Enriched Retinal Docosahexaenoic Acid and n-3 Very Long Chain Fatty Acids (VLCFA, C24-C26) on the Electroretinogram in Fat-1 Mice
Author Affiliations & Notes
  • M. Suh
    Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, Canada
  • Y. Sauve
    Ophthalmology and Physiology, University of Alberta, Edmonton, Alberta, Canada
  • C. Murray
    Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, Canada
  • J. X. Kang
    Medicine, Harvard Medical School, Charlestown, Massachusetts
  • D. W. L. Ma
    Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada
  • Footnotes
    Commercial Relationships  M. Suh, None; Y. Sauve, None; C. Murray, None; J.X. Kang, None; D.W.L. Ma, None.
  • Footnotes
    Support  Natural Sciences and Engineering Research Council of Canada (NSERC)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2848. doi:
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      M. Suh, Y. Sauve, C. Murray, J. X. Kang, D. W. L. Ma; The Effect of Enriched Retinal Docosahexaenoic Acid and n-3 Very Long Chain Fatty Acids (VLCFA, C24-C26) on the Electroretinogram in Fat-1 Mice. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2848.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Fat-1 mice can convert n-6 to n-3 fatty acids endogenously, resulting in an elevated accumulation of n-3 fatty acids in major tissues. We quantified this increase in n-3 fatty acids in the retina and examined how it might impact on retina function.

Methods: : Both wildtype (WT) and fat-1 mice were fed a modified AIN-93G diet containing 10% safflower oil for 9 weeks. Fatty acid composition of individual phospholipids was analyzed in total lipid extracts from whole eyes (except lens) with GC. Retina function was assessed with electroretinogram (ERG) recordings.

Results: : Compared to WT mice, n-3 docosahexaenoic acid (DHA, C22:6n-3) in fat-1 mice was increased 2-5 times (p<0.05) in all phospholipid classes while n-6 fatty acids was decreased by half. Novel component of retinal C32 and C34 of n-3 pentaenoic and hexaenoic VLCFA in phosphatidylcholine was significantly increased whereas n-6 tetra and pentaenoic VLCFA was depleted.Mixed scotopic a-waves (reflecting mainly rod activity) and b-waves (inner retina activity) had higher amplitudes (145% and 136%, respectively); photopic b-waves (cone mediated activity) also had elevated amplitudes (160%) in fat-1 compared with WT mice. In addition, the minimal light levels required to elicit a response (sensitivity) was higher for both rod and cone mediated responses in fat-1 compared with WT mice. Finally, glial fibrillary acidic protein (GFAP) expression (an indicator of retina stress) was increased in fat-1 mice.

Conclusions: : These findings indicate that elevated levels of DHA and n-3 VLCFA are associated with elevated amplitude of rod and cone mediated ERG. Association between GFAP expression and n-3 fatty acid content in retina needs further studies. The fat-1 mouse may be a useful model for studying therapeutic strategies to prevent and treat degenerative retinal diseases.

Keywords: nutritional factors • lipids • retina 
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