May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Evidence of a New Uveolymphatic Outflow Pathway in Human and Sheep: Implications for Aqueous Humor Drainage and Glaucoma
Author Affiliations & Notes
  • N. Gupta
    Ophthalmology & Vision Sciences, Laboratory Medicine & Pathobiology, Ophthalmic Pathology Laboratory, University of Toronto, Toronto, Ontario, Canada
    Glaucoma & Nerve Protection Unit,
    Keenan Research Centre at the Li Ka Shing Knowledge Institute of St. Michael's Hospital, Toronto, Ontario, Canada
  • M. Patel
    Keenan Research Centre at the Li Ka Shing Knowledge Institute of St. Michael's Hospital, Toronto, Ontario, Canada
  • T. Ly
    Keenan Research Centre at the Li Ka Shing Knowledge Institute of St. Michael's Hospital, Toronto, Ontario, Canada
  • E. Gümü
    Keenan Research Centre at the Li Ka Shing Knowledge Institute of St. Michael's Hospital, Toronto, Ontario, Canada
    Faculty of Medicine, Hacettepe University, Ankara, Turkey
  • M. G. Johnston
    Neuroscience, Sunnybrook Hospital Research Institute, Toronto, Ontario, Canada
  • D. Armstrong
    Neuroscience, Sunnybrook Hospital Research Institute, Toronto, Ontario, Canada
  • Y. Yucel
    Ophthalmology & Vision Sciences, Laboratory Medicine & Pathobiology, Ophthalmic Pathology Laboratory, University of Toronto, Toronto, Ontario, Canada
    Keenan Research Centre at the Li Ka Shing Knowledge Institute of St. Michael's Hospital, Toronto, Ontario, Canada
  • Footnotes
    Commercial Relationships  N. Gupta, None; M. Patel, None; T. Ly, None; E. Gümü, None; M.G. Johnston, None; D. Armstrong, None; Y. Yucel, None.
  • Footnotes
    Support  Canadian Institutes of Health Research (MJ, YY, NG), Institute of Medical Science Summer Research Program (EG), Eaton Fund (NG), Jarvis Fund (NG)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2879. doi:https://doi.org/
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      N. Gupta, M. Patel, T. Ly, E. Gümü, M. G. Johnston, D. Armstrong, Y. Yucel; Evidence of a New Uveolymphatic Outflow Pathway in Human and Sheep: Implications for Aqueous Humor Drainage and Glaucoma. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2879. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To determine whether a lymphatic circulation exists within the human and sheep ciliary body, and if present, its relationship to aqueous humor outflow.

Methods: : Twelve normal human eyes with no history of ocular disease, atherosclerosis or stroke were obtained from the Eye Bank of Canada. Human eye sections were triple-immunostained with D2-40(Signet), CD34(Abcam) and SMA(Abcam) antibodies for lymphatic vessels, blood vessels and smooth muscle cells respectively. Under general anesthesia, 50 µL fluorescent nanospheres (Invitrogen) were injected intracamerally in 3 sheep, with sacrifice 2 (n=2) and 4 (n=1) hours after the injection. Eyes were cryoprotected, serially sectioned and immunostained for a lymphatic endothelial marker (LYVE-1, Diagnostic Laboratories). Confocal laser microscopy was used to image ciliary body and capture images for 3-D construction (Microbrightfield Inc.).

Results: : In normal human eyes, numerous fine D2-40 positive lymphatic channels were observed in circular, radial and longitudinal portions of the ciliary muscle, stroma, and ciliary processes. D2-40 positive lymphatic channels surrounded many blood vessels in the ciliary body. In the normal sheep, numerous channels containing fluorescent nanospheres in the ciliary body were outlined by LVYE-1 immunoreactive lymphatic endothelial cells.

Conclusions: : A uveolymphatic pathway is described for the first time in the human and sheep ciliary body. The flow of aqueous humor into these channels indicates a new mechanism by which aqueous humor flows out of the eye, and if altered in glaucoma, is a possible novel therapeutic target.

Keywords: aqueous • outflow: ciliary muscle • ciliary body 
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