May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Role of CD147/EMMPRIN in Corneal Stroma Remodeling
Author Affiliations & Notes
  • E. E. Gabison
    Hopital Bichat AP-HP/Cornea, Fondation A de Rothschild, Paris, France
    CNRS UMR 7149, CRRET, Université Paris Val de Marne, France
  • E. Huet
    CNRS UMR 7149, CRRET, Université Paris Val de Marne, France
  • B. Vallée
    CNRS UMR 7149, CRRET, Université Paris Val de Marne, France
  • S. Menashi
    CNRS UMR 7149, CRRET, Université Paris Val de Marne, France
  • Footnotes
    Commercial Relationships  E.E. Gabison, None; E. Huet, None; B. Vallée, None; S. Menashi, None.
  • Footnotes
    Support  Fondation Avenir (study ET5-400)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2938. doi:https://doi.org/
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    • Get Citation

      E. E. Gabison, E. Huet, B. Vallée, S. Menashi; Role of CD147/EMMPRIN in Corneal Stroma Remodeling. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2938. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : We previously described EMMPRIN, the metalloproteinase inducer, in the cornea and demonstrated its up-regulation in corneal ulcerations. Recently, we demonstrated that EMMPRIN was up-regulated by TGF-b and involved in promoting fibroblast-to-myofibroblast differentiation (Huet E et al Faseb J, 2008). The aim of this study is to compare the effect of EMMPRIN and TGF-b on corneal extracellular matrix remodeling.

Methods: : EMMPRIN, collagen I, alpha-smooth muscle actin (alphaSMA) expression was analyzed in normal and ulcerated human corneas, as well as in stromal cells in culture using confoncal microscopy, immuno-blots, zymography and real-time PCR.

Results: : While EMMPRIN was predominantly expressed in the epithelium in normal corneas, its expression was markedly induced in stromal cells in ulcerated corneas in the anterior stroma, regularly co-localized with alphaSMA. In vitro, although recEMMPRIN or TGF-b treated corneal fibroblasts showed similar stimulation of alphaSMA, EMMPRIN and MMP-2, they had opposite effects on MMP-1 expression. Interestingly, recEMMPRIN did not modify TIMP-1 or type 1 collagen expression in corneal fibroblasts, whereas TGF-b treatment was associated with an increase in these molecules.

Conclusions: : TGF-b and recEMMPRIN, both involved in fibroblast-to-myofibroblast differentiation, exhibit differential effects on corneal fibroblasts collagenolytic balance. EMMPRIN up-regulation in the remodeling stroma and its regulation by TGFb and cell-cell interactions suggest that it may play an important role during corneal wound healing.

Keywords: wound healing • proteolysis • cornea: stroma and keratocytes 
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