May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Keratocyte Repopulation in UVB-Exposed Thioltransferase Knockout Mice
Author Affiliations & Notes
  • A. Podskochy
    St Erik Eye Hospital, Stockholm, Sweden
  • Y. Zhang
    St Erik Eye Hospital, Stockholm, Sweden
  • Y.-S. Ho
    Wayne State University, Detroit, Michigan
  • S. Löfgren
    St Erik Eye Hospital, Stockholm, Sweden
  • Footnotes
    Commercial Relationships  A. Podskochy, None; Y. Zhang, None; Y. Ho, None; S. Löfgren, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2944. doi:https://doi.org/
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    • Get Citation

      A. Podskochy, Y. Zhang, Y.-S. Ho, S. Löfgren; Keratocyte Repopulation in UVB-Exposed Thioltransferase Knockout Mice. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2944. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Thioltransferase is involved in cell protein homeostasis and DNA synthesis. It inhibits apoptosis and stimulates cell proliferation. Keratocyte repopulation after ultraviolet B (UVB) damage was studied in corneas of thioltransferase knockout mice.

Methods: : Six wild type mice and six thioltransferase knockout mice corneas were exposed at 300 nm UVB at a dose producing damage in the corneal stroma (8 kJ/m2). Animals were killed 3 and 7 days after exposure. Corneas were processed for light microscopy.

Results: : All corneas of wild type mice and thioltransferase knockout mice showed extensive damage 3 days after UVB exposure. Keratocytes disappeared throughout the entire thickness of the UVB-damaged central stroma. Corneal thickness was nearly doubled compared with non-treated control corneas. However, 7 days after UVB exposure corneas of wild type mice were almost completely repopulated by keratocytes, only superficial ¼ of the stroma was still free of keratocytes. Corneal thickness was almost normal. Corneal stroma in the thioltransferase knockout mice 7 days after UVB exposure was still not repopulated by keratocytes and the corneas were still very thick.

Conclusions: : The keratocyte repopulation in thioltransferase knockout mice is delayed. Thioltransferase seems to play an important role in the corneal wound healing and keratocyte repopulation after UVB induced damage.

Keywords: cornea: basic science • cornea: stroma and keratocytes • wound healing 
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