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A. Podskochy, Y. Zhang, Y.-S. Ho, S. Löfgren; Keratocyte Repopulation in UVB-Exposed Thioltransferase Knockout Mice. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2944.
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Thioltransferase is involved in cell protein homeostasis and DNA synthesis. It inhibits apoptosis and stimulates cell proliferation. Keratocyte repopulation after ultraviolet B (UVB) damage was studied in corneas of thioltransferase knockout mice.
Six wild type mice and six thioltransferase knockout mice corneas were exposed at 300 nm UVB at a dose producing damage in the corneal stroma (8 kJ/m2). Animals were killed 3 and 7 days after exposure. Corneas were processed for light microscopy.
All corneas of wild type mice and thioltransferase knockout mice showed extensive damage 3 days after UVB exposure. Keratocytes disappeared throughout the entire thickness of the UVB-damaged central stroma. Corneal thickness was nearly doubled compared with non-treated control corneas. However, 7 days after UVB exposure corneas of wild type mice were almost completely repopulated by keratocytes, only superficial ¼ of the stroma was still free of keratocytes. Corneal thickness was almost normal. Corneal stroma in the thioltransferase knockout mice 7 days after UVB exposure was still not repopulated by keratocytes and the corneas were still very thick.
The keratocyte repopulation in thioltransferase knockout mice is delayed. Thioltransferase seems to play an important role in the corneal wound healing and keratocyte repopulation after UVB induced damage.
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