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Y. Wang; The Effect of EGF on the Proliferation of Rabbit Corneal Epithelial Cells and the Expression of the Cell Cycle-Regulatory Proteins. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2953.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the effect of epidermal growth factor (EGF) on the proliferation of cultured rabbit corneal epithelial (RCE) cells and the expression of cell cycle-regulatory proteins.
Cultured RCE cells were exposed to EGF at different doses (0,1,5,10,25,50 ng/ml) and different time(0,2,4,6,8,10 days). Cell morphologic changes were observed under a phase contrast microscope. Cell proliferation was measured using the WST-1 cell proliferation assay and the expression of cell cycle-regulatory proteins, CyclinD1, CDK4, p27, p21 and p18, was examined using Western blot analysis.
The cell morphology had no obvious change after treatment with EGF, but EGF significantly induced the proliferation of RCE cells in a time-dependent manner. In the different dose of EGF treatment groups,the OD values at 72 h are higher than those in the control groups(P < 0.01), and 10 ng/ ml of EGF maximally stimulated the proliferation of RCE. Protein expression of CyclinD1 and CDK4 was markedly increased after EGF treatment. Furthermore, p27 protein expression was significantly inhibited by EGF. However, there was no detectable difference in p21 and p18 protein expression between EGF treatment and control groups.
This study suggested that EGF could induce RCE cell proliferation, and the up-regulation of CyclinD1 and CDK4 as well as the down-regulation of p27 might account for this induced proliferation.
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