The Effect of Topical Application of Cyclosporine on the Mrna Expression of Tlr 4 and Pro-Inflammatory Cytokines in the Cornea of Lps Injected Mice

Abstract

Purpose: : To investigate the effect of topical application of cyclosporine on the expression of TLR 4 and pro-inflammatory cytokines in the cornea after LPS was injected in mice

Methods: : 12 C5BL6 mice were divided into 4 groups. 0.2cc normal saline was injected at subconjunctiva in negative control prior to instillation of topical normal saline (NS). 4ug/0.2cc LPS was injected subconjunctivally immediately before instillation of NS in positive control or 0.05%- , 0.5%- cyclosporine applied group . Topical cyclosporine or NS for control were applied every 15 minutes for four times and every 1hour for two times for loading, and then they were applied every 2 hours for 24 hours. RT- PCR was done for TLR2, 4, IL-1a, MCP, RANTES, IL-6 and Interferon (IFN)-gamma-inducible protein (IP-10).

Results: : RT-PCR analysis revealed that there were no significant differences of TLR2 or 4 in all groups. mRNA expression of IL-1a, RANTES, and IP-10 were significantly decreased in 0.05%- , 0.5%- cyclosporine applied group.

Conclusions: : Topical application of cyclosporine reduces expression of proinflammatory cytokines of innate immunity on the cornea, suggesting that it can be useful in blepharitis associated keratitis.

Keywords: immunomodulation/immunoregulation • cornea: basic science • signal transduction