May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
The Effect of Topical Application of Cyclosporine on the Mrna Expression of Tlr 4 and Pro-Inflammatory Cytokines in the Cornea of Lps Injected Mice
Author Affiliations & Notes
  • S. Park
    Deptment of Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
    Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • J. Jeong
    Deptment of Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
    Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • M. Shin
    Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • H. Lee
    Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • W. Wee
    Deptment of Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
    Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • J. Lee
    Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
    Deptment of Ophthalmology, Seoul National University Bundang Hospital, Seongnam Si Bundang-gu, Gyeonggi-Do, Republic of Korea
  • M. Kim
    Deptment of Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
    Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  S. Park, None; J. Jeong, None; M. Shin, None; H. Lee, None; W. Wee, None; J. Lee, None; M. Kim, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 2954. doi:https://doi.org/
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      S. Park, J. Jeong, M. Shin, H. Lee, W. Wee, J. Lee, M. Kim; The Effect of Topical Application of Cyclosporine on the Mrna Expression of Tlr 4 and Pro-Inflammatory Cytokines in the Cornea of Lps Injected Mice. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2954. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the effect of topical application of cyclosporine on the expression of TLR 4 and pro-inflammatory cytokines in the cornea after LPS was injected in mice

Methods: : 12 C5BL6 mice were divided into 4 groups. 0.2cc normal saline was injected at subconjunctiva in negative control prior to instillation of topical normal saline (NS). 4ug/0.2cc LPS was injected subconjunctivally immediately before instillation of NS in positive control or 0.05%- , 0.5%- cyclosporine applied group . Topical cyclosporine or NS for control were applied every 15 minutes for four times and every 1hour for two times for loading, and then they were applied every 2 hours for 24 hours. RT- PCR was done for TLR2, 4, IL-1a, MCP, RANTES, IL-6 and Interferon (IFN)-gamma-inducible protein (IP-10).

Results: : RT-PCR analysis revealed that there were no significant differences of TLR2 or 4 in all groups. mRNA expression of IL-1a, RANTES, and IP-10 were significantly decreased in 0.05%- , 0.5%- cyclosporine applied group.

Conclusions: : Topical application of cyclosporine reduces expression of proinflammatory cytokines of innate immunity on the cornea, suggesting that it can be useful in blepharitis associated keratitis.

Keywords: immunomodulation/immunoregulation • cornea: basic science • signal transduction 
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