Purchase this article with an account.
S. Park, J. Jeong, M. Shin, H. Lee, W. Wee, J. Lee, M. Kim; The Effect of Topical Application of Cyclosporine on the Mrna Expression of Tlr 4 and Pro-Inflammatory Cytokines in the Cornea of Lps Injected Mice. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2954. doi: https://doi.org/.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To investigate the effect of topical application of cyclosporine on the expression of TLR 4 and pro-inflammatory cytokines in the cornea after LPS was injected in mice
12 C5BL6 mice were divided into 4 groups. 0.2cc normal saline was injected at subconjunctiva in negative control prior to instillation of topical normal saline (NS). 4ug/0.2cc LPS was injected subconjunctivally immediately before instillation of NS in positive control or 0.05%- , 0.5%- cyclosporine applied group . Topical cyclosporine or NS for control were applied every 15 minutes for four times and every 1hour for two times for loading, and then they were applied every 2 hours for 24 hours. RT- PCR was done for TLR2, 4, IL-1a, MCP, RANTES, IL-6 and Interferon (IFN)-gamma-inducible protein (IP-10).
RT-PCR analysis revealed that there were no significant differences of TLR2 or 4 in all groups. mRNA expression of IL-1a, RANTES, and IP-10 were significantly decreased in 0.05%- , 0.5%- cyclosporine applied group.
Topical application of cyclosporine reduces expression of proinflammatory cytokines of innate immunity on the cornea, suggesting that it can be useful in blepharitis associated keratitis.
This PDF is available to Subscribers Only