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S. Park, J. Jeong, M. Shin, H. Lee, W. Wee, J. Lee, M. Kim; The Effect of Topical Application of Cyclosporine on the Mrna Expression of Tlr 4 and Pro-Inflammatory Cytokines in the Cornea of Lps Injected Mice. Invest. Ophthalmol. Vis. Sci. 2008;49(13):2954.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the effect of topical application of cyclosporine on the expression of TLR 4 and pro-inflammatory cytokines in the cornea after LPS was injected in mice
12 C5BL6 mice were divided into 4 groups. 0.2cc normal saline was injected at subconjunctiva in negative control prior to instillation of topical normal saline (NS). 4ug/0.2cc LPS was injected subconjunctivally immediately before instillation of NS in positive control or 0.05%- , 0.5%- cyclosporine applied group . Topical cyclosporine or NS for control were applied every 15 minutes for four times and every 1hour for two times for loading, and then they were applied every 2 hours for 24 hours. RT- PCR was done for TLR2, 4, IL-1a, MCP, RANTES, IL-6 and Interferon (IFN)-gamma-inducible protein (IP-10).
RT-PCR analysis revealed that there were no significant differences of TLR2 or 4 in all groups. mRNA expression of IL-1a, RANTES, and IP-10 were significantly decreased in 0.05%- , 0.5%- cyclosporine applied group.
Topical application of cyclosporine reduces expression of proinflammatory cytokines of innate immunity on the cornea, suggesting that it can be useful in blepharitis associated keratitis.
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