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S. Shin, K. M. Bumsted O’Brien; Where Are the Retinal Progenitor Cells of the Chicken Rod Free Area Centralis Located in the Developing Optic Vesicle?. Invest. Ophthalmol. Vis. Sci. 2008;49(13):3090.
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© ARVO (1962-2015); The Authors (2016-present)
To determine the embryonic origin of the chicken rod free area centralis by investigating the location of its progenitor cells in the developing optic vesicle by cell lineage analysis and examining the amount of plasticity in regenerating the rod free zone after varying degrees of optic vesicle ablation.
DiI crystals were implanted into the developing chicken optic vesicle (HH 10-11) to trace cell lineage. In a second set of experiments, varying amounts of the optic vesicle distal tip were ablated and then DiI was applied either on the dorsal, ventral, anterior or posterior edge of the wound to determine the location of the regenerative region. The embryos from both experiments were allowed to develop until embryonic day (E) 6.5 - 7. Eyes were enucleated, fixed in 4% paraformaldehyde, and the location of the DiI labeling was traced. The retinas were then processed for wholemount in situ hybridization using DIG labeled visinin RNA probes, which correlates with the location of the rod free zone prior to the expression of rod opsin.
Injections performed at the distal tip of the optic vesicle showed expression in the central retina above the optic nerve head. DiI labeling of a region slightly dorsal and anterior to the optic vesicle distal tip correlated with the location of the developing rod free zone. When a small amount of optic vesicle was ablated (less than 20%), the rod free zone was present, and if more than 20% was ablated the rod free zone was lost. More than 50% removal of retinal progenitor cells did not allow normal eye development.
These observations suggest that the chicken area centralis originates from cells located near, but not at the distal tip of the developing optic vesicle. The data also suggest a minimum amount of plasticity in the immediately surrounding optic vesicle regions which allows for the regeneration of the rod free zone.
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